We describe a fast light microscopic procedure for the simultaneous enzyme cytochemical detection of three different DNA target sequences in contrasting colors in both interphase and metaphase cell preparations. Chromosome-specific DNA probes labeled with either biotin, digoxygenin, or fluorescein were hybridized as a mixture and detected clearly and accurately by precipitates of the horseradis...

Induction and secretion of acid phosphatases (APases) is considered to be an important strategy for improving plant growth under conditions of low inorganic phosphate (Pi). Purple acid phosphatases (PAPs), are an important class of plant APases that could be secreted into the rhizosphere to utilize organic phosphorus (Po) for plant growth and development. To date, only a few members of the PAP ...

The nucleotide sequence of the PH05 gene of the yeast, Saccharomyces cerevlelae, which encodes repressible acid phosphatase (APase) was determined. Comparison of N-terainal amino acid sequence deduced from the nucleotide sequence with that of the purified repressible APase revealed the existence of a putative signal peptide in the precursor protein. The signal peptide was shown to contain 17 am...

A secreted phosphodiesterase/alkaline phosphatase, APaseD, was purified from a culture of Bacillus subtilis JH646MS. Its phosphodiesterase activity was reminiscent of an APase isolated and characterized previously. Immunoassay and N-terminal sequencing showed the two proteins to be identical. Using the first 20 amino acids of the mature protein, a BLAST search of GenBank was used to find an hom...

Continuous tissue culture lines derived from the bone marrow or peripheral blood of normal humans or patients with leukemia frequently show Epstein-Barr virus nuclear antigen (EBNA) and surface immunoglobulins (SIg), characteristics of transformed B lymphocytes. A survey of myeloid cell-associated enzymes produced by 18 EBNA-positive lines revealed leukocyte alkaline phosphatase (LAP), myeloide...