The human promyelocytic leukemic cell line HL-60 can be induced to mature monocytes and macrophages in vitro by lymphocyte-conditioned medium. We are reporting sequential changes in surface antigenic expressions, which are sensitive markers of the characteristic events in the process of cell differentiation. The promyelocyte membrane antigen, detected by a monoclonal antibody produced using HL-...

The human promyelocytic leukemic cell line HL-60 can be induced to mature monocytes and macrophages in vitro by lymphocyte-conditioned medium. We are reporting sequential changes in surface antigenic expressions, which are sensitive markers of the characteristic events in the process of cell differ entiation. The promyelocyte membrane antigen, detected by a monoclonal antibody produced using HL...

The human leukemic cell lines, K562, KG-1, and HL-60, and the blast subclones, KG-1a and HL-60 blast, were utilized to relate differences in nonhistone protein antigens to stages of myeloid cell differentiation. Chromatin proteins were separated on SDS-polyacrylamide gels, transferred electrophoretically to nitrocellulose sheets, and visualized by the peroxidase-antiperoxidase method of Sternbe...

Neisseria meningitidis type B is a major world-health problem. The Meningococcus type B capsular polysaccharide (MnB) is very poorly immunogenic and no vaccine to the antigen exists. Here, we conjugated the MnB to a T-cell carrier peptide (p458) derived from the self-60kDa heat shock protein molecule. The conjugate vaccine was effective in inducing long-lasting IgG antibodies to the MnB antigen...