Bacterial abundance, biomass, cell volume, and morphological diversity were studied in sediment samples collected from the coastal area of the eastern mid Adriatic Sea. Samples from the topmost 10 cm of sediment were taken monthly from January to December 2002. The number of bacteria varied from 3.54 x 109 cells g–1 in July to 8.08 x 109 cells g–1 in September. Biomass was lowest in May (78 μg ...

Evolutionary genetics studies the patterns and mechanisms of genetic changes underlying evolutionary change. Because biological diversity at all levels (including population, species, and ecosystem diversity) has a genetic basis and is a result of evolution, evolutionary genetics provides insight into the ultimate mechanisms creating biodiversity. Evolutionary genetics utilizes empirical tools ...

The present work describes the construction and validation of a human scFv library with a novel design approach to synthetic complementarity determining region (CDR) diversification. The advantage of synthetic antibody libraries includes the possibility of exerting fine control over factors like framework sequences, amino acid and codon usage, and CDR diversity. However, random combinatorial sy...

Antibody technologies are the most representative success-case in biopharmaceutical industry. Widely available purification fail providing a dedicated universal platform that can accommodate antibodies structural diversity, namely from non-human sources, as chicken IgY, and antigen-binding fragments. In this work, we took inspiration natural engineered antibody-binding ligands, to rationally de...

The great diversity of arthropod body plans, together with our detailed understanding of fruit fly development, makes arthropods a premier taxon for examining the evolutionary diversification of developmental patterns and hence the diversity of extant life. Crustaceans, in particular, show a remarkable range of morphologies and provide a useful outgroup to the insects. The amphipod crustacean P...

Lymphocytes from eight individuals out of 60 healthy donors, whose plasmas showed relatively higher antibody titer for a target antigen of death receptor 5 (DR5), were selected for the source of antibody genes to construct so called an anti-DR5 pseudo-immune human single-chain fragment variable (scFv) library on the yeast cell surface (approximately 2x10(6) diversity). Compared with a large non...