Diisopropyl fluorophosphate (DFP) is a highly specific inactivator of e&erases, such as cholinesterase and chymotrypsin (2), and combines irreversibly with these enzymes (3,4). In a previous report from this laboratory (5) we have shown that, when the reaction product of DFP and chymotrypsin, namely, diisopropylphosphoryl chymotrypsin, is partially hydrolyzed, serine phosphoric acid may be obta...

S EVERAL SUGGESTIONS have been made to explain the parallelism between serum cholinesterase and serum albuniin levels in chronic liver disease-first recognized by Faber (i), and since, by many othersand the moderate fall in serum cholinesterase found to occur ill normal persons after the infusion of albumin (2). Kunkel and Ward (3) suggested that a normal serum albumin level was needed for the ...

Pancuronium causes a powerful and highly selective inhibition of human serum cholinesterase in vitro. The inhibition was studied in serum from 14 individuals of both sexes (5-60 years of age) with normal reactions to suxamethonium. Pancuronium, in a concentration of 2.3 x 10(-7) M, caused a 50% inhibition of the enzymatic hydrolysis of acetylcholine, when this substrate was present in a concen...

The aims of this study were to investigate the presence of different esterase activities in plasma and liver for Japanese quail and to combine determination of both carboxylesterase and cholinesterase as biochemical biomarker in order to identify the effects of carbamate and organophosphate compounds exposure. Carboxylesterase exhibits larger sensitivity to carbamate and organophosphate compoun...

Human serum cholinesterase was digested with pepsin under conditions which left disulfide bonds intact. Peptides were isolated by high pressure liquid chromatography, and those containing disulfide bonds were identified by a color assay. Peptides were characterized by amino acid sequencing and composition analysis. Human serum cholinesterase contains 8 half-cystines in each subunit of 574 amino...

A cholinesterase was purified 36-fold from mung bean (Phaseolus aureus) roots by a combination of differential extraction media and gel filtration. The enzyme could be effectively extracted only by high salt concentration, indicating that it is probably membrane-bound. Methods used for assaying animal cholinesterases were tested, two of which were adapted for use with the bean cholinesterase. T...

the crude methanolic extract of the seeds of celastrus paniculatus along with its organic soluble fractions were tested for their possible antioxidant and anti-alzheirmer (ad) activity. the extracts showed prominent dpph free radical scavenging activity, inhibiting activity of authentic peroxynitrite (onoo-) and inhibition of total reactive oxygen species (ros) generation. in dpph radical scave...

background: treatment of chronic constipation is creating one of the major problems for doctors and patients. pyridostigmine increases the gastrointestinal motility through the effects on cholinesterase. it seems that this mechanism can reduce chronic constipation. the aim of this study was to compare the effects of pyridostigmine and bisacodyl on chronic constipation. methods: this study was c...

Patients with Alzheimer's disease after an initial response to cholinesterase inhibitors may complain a later lack of efficacy. This, in association with incident neuropsychiatric symptoms, may worsen patient quality of life. Thus, the switch to another cholinesterase inhibitor could represent a valid therapeutic strategy. The aim of this study was to investigate the effectiveness of the switch...

This report assesses the value of cholinesterase (ChE) activity as an indicator of liver function and analyses its usefulness in the diagnosis of jaundice. A rapid method of determination has been used, and alterations in ChE activity in conditions other than liver disease have been studied to permit a fuller and more accurate survey of its value as a liver function test. The level of serum cho...