The aim of this research was to develop a quantitative sandwich-strip ELISA developed on nitrocellulose membrane with anti-H alpha and anti-hCG beta antibodies. detection antibody conjugated horseradish peroxidase, luminol substrate following optimization hCG. Concentration hCG in the BF determined for 101 embryo biopsies (Study 1) 96 2) respectively, results were compared previously recorded m...

The detection of anti-hepatitis A virus (HAV) antibody levels by diagnostic kits in the convalescent period of disease generally use immunoglobulin G (IgG), which is expensive. An alternative to IgG is immunoglobulin Y (IgY), an immunoglobulin antibody encountered in birds and reptiles. The aim of this study was to develop a competitive immunoenzymatic assay to measure total anti-HAV antibody l...

Materials Thrombin (bovine plasma, 1880 NIH units/mg protein) was purchased from Sigma (St. Louis, MO, U.S.A.). Simvastatin, fluvastatin and pravastatin were supplied by Merck & Co. (Rahway, N.J., U.S.A.), Novartis Pharma Inc. (Tokyo, Japan) and Sankyo Co. (Tokyo, Japan), respectively. TFLLR-NH2 (PAR1-activating peptide) and GYPGKFNH2 (PAR4-activating peptide) were purchased from Bachem (Bubend...

BACKGROUND Vitiligo has been associated with multiple endocrine and immune conditions. Several laboratory tests have been assessed in this disease with controversial results. OBJECTIVE The aim of this study is to analyze the levels autoantibodies, basal glycaemia, vitamin B12, folic acid and thyroid function and its association with the diagnosis and outcome of vitiligo patients through a cas...

An improved enzyme-linked immunosorbent assay (ELISA) for detection of heterophile Hanganutziu-Deicher (HD) antibodies and antigens, which are frequently detected in sera and/or cancerous tissues from patients with various cancers was developed using biotinylated chicken anti-GM3(NeuGc) antibody and avidin-horseradish peroxidase conjugate. The N-glycolylneuraminyllactosyl-ceramide, GM3(NeuGc) g...

Background: Premature ovarian failure (POF) is a disorder of multi causal etiology. Autoimmunity has been proposed as a mechanism for some cases of ovarian follicle dysfunction which is evident in POF. The aim of this study was to identify the level of auto-antibodies in POF and familial POF patients. Materials and Methods: In this study, auto-antibodies including anti-ovarian antibody (AOA), a...

A dot immunobinding assay ( DIA) was used for a quantitative and qualitative assay of rubella antibody. Purified antigen and conjugated anti-human immunoglobulin (RAHlg) were prepared. Nitrocellulose paper dotted with the antigen was added to serially diluted sera or blood samples. The reacting antibodies were visualized by a peroxidase system. Development of a colored insoluble substrate ...

Background: Abortion is the interruption and/or termination of pregnancy, either spontaneously or intentionally, before fetus develops sufficiently to survive. About 80% abortions occur in first 12 weeks pregnancy. The underlying mechanism about half cases involves chromosomal abnormalities. Objective: To evaluate association between TSH, T3, T4, anti-thyroglobulin antibody and thyroid peroxida...

Hashimoto's encephalopathy (HE) is a steroid-responsive relapsing neuropsychiatric disorder associated with high titers of antithyroid antibody with or without thyroid dysfunction. We report a case of HE in a 78 year old female who developed sudden onset behavioral abnormalities associated with choreiform movement of extremities. All causes of vascular, infective, metabolic, autoimmune, paraneo...