BACKGROUND AND AIMS Free-hand sectioning of living plant tissues allows fast microscopic observation of internal structures. The aim of this study was to improve the quality of preparations from roots with suberized cell walls. A whole-mount procedure that enables visualization of exo- and endodermal cells along the root axis was also established. METHODS Free-hand sections were cleared with ...

The impact of sperm DNA fragmentation on assisted reproductive technology (ART) successes, in terms of outcome, is now established. High levels of DNA strand breaks severely affect the probability of pregnancy. The importance of sperm nucleus condensation in early embryogenesis and, subsequently, on the quality of the conceptus is now emerging. In this article we have compared in situ analyses ...

We report a case of subcutaneous infection in a 55-yr-old Korean diabetic patient who presented with a cystic mass of the ankle. Black fungal colonies were observed after culturing on blood and Sabouraud dextrose agar. On microscopic observation, septated ellipsoidal or cylindrical conidia accumulating on an annellide were visualized after staining with lactophenol cotton blue. The organism was...

background: diabetes mellitus (dm), primary or idiopathic is a chronic disorder of the carbohydrate, lipid and protein metabolism. dm may impact male reproductive function at several levels. it is shown that dm has detrimental effects on sperm parameters in human and experimental animals. objective: the aim of this study was to observe the effects of diabetes on sperm parameters (viability, cou...

introduction: the aim of this study was to evaluate the effect of sperm chromatin anomalies on fertilization outcome post icsi. material and methods: therefore along with semen parameters, cma3 staining for protamine deficiency, aniline blue staining for excessive histones, sds for sperm chromatin stability and sds+edta for ability of sperm to undergo decondensation were carried out on 55 semen...

Background: Plant growth regulators gave entry into animals and human cells through diet. Gibberellic acid (GA3) is an extensively prevalent plant growth regulator due to its use in agriculture. Therefore current study was designed to investigate the effect of GA3 compound on sperm DNA integrity, nuclear maturity and motility. Materials and Methods: To follow-up current study 12 mature male s r...

Dark blue rings and circles emerged when the non-specific polysaccharide stain lactophenol cotton blue was added to Gram stained slides. The dark blue staining is attributable to the presence of capsular polysaccharides and bacterial slime associated with clumps of Gram-negative bacteria.  Since all bacterial cells are glycosylated and concentrate polysaccharides from the media, the majority of...