the effect of osmolarity and glycerol different levels in soybean lecithin-based extender on the bull sperm quality after cryopreservation was examined using six holstein bulls in a 2 × 3 factorial trial based on completely randomized design, with three levels of osmolarity (250, 300 and 350 mosml) and two levels of glycerol (5 and 7 %) .in general, semen samples were collected 36 times (from e...

The present study was undertaken to investigate the effect of various levels of vitamin B12 in Tris extender on semen quality of Dallagh rams following the cooling and freeze/thawing process. Semen was collected from six healthy and mature rams with an average body weight of 60.0 ± 5.0 Kg using an electro ejaculator. High quality samples were mixed and diluted in Tris extender supplemented with...

Two hours after semen collection, aliquots containing 6xlO 9 spermatozoa were centrifuged for 10 rain at 300 g. The seminal plasma was removed and the spermatozoa were resuspended to 5 ml with Beltsville F5 (BF5) extender. The semen was cooled gradually to 5 C over a 2-hr period, 5 ml of BF5 containing 2% glycerol was added and the semen was mixed and frozen immediately into pellets of 0.15 to ...

The main purpose of this study was to evaluate the influence of the photoperiod on the seminal traits of crossbreed wool-producing rams throughout on year period. For the effect of photoperiod two periods were considered: decreasing daylight length (summer and autumn) and increasing daylight length (winter and spring). For the study 5 Baluchi × Moghani (BL×MG) and 5 Arkharmerino × Moghani (AM×M...

In the first experiment, osmotic pressure of semen and seminal plasma in a semen sample from each of the 20 mature Nili-Ravi buffalo bulls was determined. In the second experiment, effects of osmotic pressure on motility (%), plasma membrane integrity (%) and viability (%) in fresh and frozen-thawed semen samples from each of the seven mature Nili-Ravi buffalo bulls was determined. In the first...

This study was conducted to assess the post thaw quality of Kundhi buffalo semen diluted in tris based diluents. After collection the ejaculates were pooled and checked for color, volume, pH, mass activity, progressive linear motility% (PLM), and intactness of cell membrane. The semen qualifying these tests was divided into four aliquots A, B, C and D, diluted with tris based diluents; the last...

The objective of this study was to determine the effects of thawing groups of 2, 5, 10, 15, or 20 .5-ml French straws on post-thaw spermatozoal viability. Thermostatically controlled and nonthermostatically controlled thawing baths were compared. Using a split-plot design, semen from 10 bulls was extended in egg yolk citrate, frozen, and then thawed (in the respective groups) at 36 degrees C in...

BACKGROUND Artificial insemination (AI) using frozen-thawed semen is well established and routinely used for breeding in various mammalian species. However, there is no report of the birth of elephant calves following AI with frozen-thawed semen. The objective of the present study was to investigate the fertilizing ability of chilled and frozen-thawed semen in the Asian elephant following artif...

This study was aimed to investigate the effects of omega-3, 6, 9 fatty acids on the characteristics of bovine chilled and frozen-thawed semen. For this purpose, oil containing different levels of omega-3, 6, 9 fatty acids were added to semen extender. To emulsify the oil in semen extender, polyethylene glycol (PEG) was added as a suitable solvent and the solution was finally sonicated. Five pro...