In tro duc tion: Co bas 6000 (Roc he, Ger ma ny) is bioc he mis try ana lyzer for spec trop ho to met ric, im mu no tur bi di met ric and io n-se lec ti ve de ter mi nation of bioc he mi cal ana lytes. He re by we pre se nt ana lytical va li da tion wi th em pha sis on met hod per for man ce jud gme nt for rou ti ne ope ra tion. Ma te ria ls and met ho ds: Va li da tion was ma de for 30 ana lyt...

Antinuclear antibodies (ANA) were found in 54 (7.0%) out of 767 treated hypertensive patients compared with 59 (2.4%) out of 2470 healthy controls. Inclusion of a non-practolol beta-blocker in the treatment regimen did not significantly affect the incidence of ANA. ANA was found in significantly more patients being treated with methyldopa (13.0%) than patients receiving other hypotensive agents...

Francieli Barreiro Ribeiro, Cleliani de Cássia da Silva, Ana Carolina Junqueira Vasques, Mariana Porto Zambon, Ana Maria De Bernardi Rodrigues, Daniella Fernandes Camilo, Maria Ângela Reis de Góes Monteiro Antonio, Bruno Geloneze Neto, Francieli Barreiro Ribeiro, Cleliani de Cássia da Silva, Ana Carolina Junqueira Vasques, Mariana Porto Zambon, Ana Maria De Bernardi Rodrigues, Daniella Fernande...

recently, using polymerase chain reaction (pcr) technique, the dna of cytomegalovirus was detected from depigmented white patches in patients with vitiligo. the reactivation of infection and occasional anti-cmv igm circulating among patients infected by cytomegalovirus has been reported for years. we have studied 26 patients with clinical signs and symptoms of vitiligo, some of whom had a histo...

background: apart from genetic and environmental factors, activation of autoreactive mechanisms has been proposed to play a role in the pathogenesis of schizophrenia. in re-cent years, considerable work has been carried out to understand the role and contribution of the immune system in this disease. objective: to investigate the t cell response to phytohaemagglutinin (pha) and determine the se...

BACKGROUND/AIMS The findings of several recent studies suggest that antinuclear antibodies (ANAs) are frequently detected in patients with acute hepatitis A (AHA). However, the clinical significance of a positive ANA test remains uncertain. This study was performed to evaluate the clinical significance of ANAs in AHA patients. METHODS All patients admitted with AHA were consecutively enrolled...

BACKGROUND The referral of patients with positive anti-nuclear antibody (ANA) tests has been criticized as an inappropriate use of medical resources. The utility of a positive ANA test in a central triage (CT) system was studied by determining the autoantibody profiles and clinical diagnoses of patients referred to rheumatologists through a CT system because of a positive ANA test. METHODS Pa...

OBJECTIVE To provide data on (a) the probability of detecting antinuclear antibodies (ANA) in a large and consecutive cohort of serum samples referred for ANA testing and (b) the probability of detecting more specific antinuclear reactivities (anti-DNA and anti-extractable nuclear antigens (anti-ENA)) in serum samples with a positive screening test (indirect immunofluorescence on HEp-2 cells). ...

Virgínia Oliveira Fernandes, Ana Paula Dias Rangel Montenegro, Clarisse Mourão Melo Ponte, Lia Beatriz de Azevedo Souza Karbage, Manuela Montenegro Dias de Carvalho, Daniel Duarte Gadelha, Synara Cavalcante Lopes, Marivaldo Loyola Aragão, Ana Paula Abreu Martins Sales, Cristiane Bezerra Rocha Liberato, Ana Gardênia Liberato Ponte Farias, Catarina Brasil D’Alva, Francisco Herlânio Costa Carvalho...

In situ fabricated nucleic acids microarrays are versatile and very high-throughput platforms for aptamer optimization and discovery, but the chemical space that can be probed against a given target has largely been confined to DNA, while RNA and non-natural nucleic acid microarrays are still an essentially uncharted territory. 2΄-Fluoroarabinonucleic acid (2΄F-ANA) is a prime candidate for suc...