The present study was aimed to isolate, identify and screening of enzyme producing bacteria from soil samples. For this purpose the soil samples were collected from agriculture fields at sangam dairy, Guntur district, fish culture ponds, timber depot soil at rural areas of Bhimavaram, West Godavari district and oil mills at Vijayawada, Krishna district, Andhra Pradesh. A total of seventeen stra...

The desert, which covers around one-third of Earth’s continental surface, is defined as the harshest terrestrial environment and comprises a highly extensive biome ecosystem. Microorganisms are key drivers that maintain integrity desert ecosystems. Over past few decades, desertification has increased owing to changes in rainfall patterns global warming, characterized by land degradation, loss m...

Pullulanase. a debranching enzyme that specifically cleaves a 1.6 linkages in amylopectin and pullulan, differs from a-amylase which hydrolyses the a 1,4 linkages of starch, amylose and amylopectin [ I ] . Pullulanases are inactive against linear a 1,4 linkages and a-amylases do not hydrolyse pullulan. Some novel amylolytic enzymes capable of hydrolysing both starch and pullulan have been disco...

this research has focused on isolation and characterization of a strain of bacillus sp. from alkaline soil, which was able to produce extracellular alkaline protease at phs ranging from 8 to 11 and temperatures of 20 to 50ºc. also the impact of different carbon andnitrogen sources were investigated. the yield and fold of enzyme purification was 24% and 50 times, respectively. molecular weight o...

Abstract The effects of Calcium (Ca+2) on virulence and some parameters should be analyzed in this study. Pseudomonas aeruginosa Gram (-) Bacillus cereus (+) were used. Both bacteria are soil bacteria. In study; the effect Ca+2 protease, amylase, LasB elastolytic assay, H2O2, pyorubin biofilm metabolites these investigated during 24 hour time. study, production secondary P. B. was presented for...

Thermophilic bacteria are among the truly unique microorganisms inhabiting our unicellular kingdom. Attempts to explain their ability to proliferate at temperatures over 70 C have resulted in widespread investigation during the past two decades. (The precise upper temperature limit for bacterial growth is a controversial issue. For a recent discussion of this problem, see reference 10.) The lit...

The specificity of Bacillus stearothermophilus TRS40 neopullulanase toward amylose and amylopectin was analyzed. Although this neopullulanase completely hydrolyzed amylose to produce maltose as the main product, it scarcely hydrolyzed amylopectin. The molecular mass of amylopectin was decreased by only one order of magnitude, from approximately 10(8) to 10(7) Da. Furthermore, this neopullulanas...

The mean amylase level in 42 human bile samples was 154 IU/l and there was no significant difference in the amylase activity of 32 paired serum and bile samples. Estimation of the amylase thermolability of bile showed it to be similar to that of serum. This suggests that the amylase activity in bile may have filtered through the liver from the hepatic circulation rather than refluxed from the p...