A protein activator (GMz-activator) specific for stimulating the hydrolysis of G M ~ ganglioside (GalNAcb1 4GalC3 + 2aNeuAc]/?l-+ 4Glcb1-+ 1’Cer) by &hexosaminidase A has been purified over 1OS-fold with a high yield from human liver. The purification procedure includes the adjustment of the pH of liver extract to pH 4.3, followed by ammonium sulfate precipitation, Sephadex G-200 filtration, an...

The activation and catalytic mechanism of corn mitochondrial F(1) were examined for the two distinct forms of the enzyme which appear upon storage in ammonium sulfate or glycerol. Apparently irreversible differences in the stability of the two active forms were found. Nucleosidetriphosphate induced activation of the enzyme was found to produce lasting effects on subsequent catalysis. These effe...

Fawsitt (1) and Werner (2) have studied the hydrolysis of urea by acids and by alkalies. They agreed in assuming that cyanate was an intermediate in the hydrolysis, but differed widely as to the details of the mechanism and as to experimental values for the velocity constants of the reactions involved.1 The reverse reaction, the synthesis of urea from ammonia and cyanate, has been investigated ...

Oat (Avena sativa cv Fulghum) fructan hydrolase was purified by ammonium sulfate precipitation and anion-exchange, hydrophobic interaction, and size-exclusion chromatography. The enzyme was purified to homogeneity as determined by the presence of a single band (43 kD) on a silver-stained sodium dodecyl sulfate-polyacrylamide gel. A mixture of /3-2,6-linked fructan (neokestin) isolated from oat ...

During studies on photophosphorylation in cell-free preparations of the blue-green alga, Anabaena variabilis, a photohydrolysis of ATP was found to be induced by high concentrations of cysteine or GSH, while photophosphorylation became inhibited (1, 2). A similar photohydrolysis was also reported with chloroplasts from spinach leaves, but still higher sulfhydryl concentrations were required to ...

A sulfur-containing glycolipid, accounting for ca. 25% of the total polar lipids, has been isolated from the extreme halophile Halobacterium cutirubrum. The ammonium salt of the lipid was found to have the molecular formula C(61)H(117)O(21)S.NH(4), and on strong acid hydrolysis it yielded 2,3-di-O-phytanyl-sn-glycerol, glucose, mannose, galactose, and sulfate in equimolar proportions. Infrared ...

Enzymatic hydrolysis is a key step in bioethanol production. Efficient hydrolysis requires a consortium of different enzymes that are able to hydrolyze cellulose and hemicellulose into fermentable sugars. Myceliopthora thermophila is a promising candidate for the production of thermophilic cellulolytic enzymes, the use of which could reduce the cost of ethanol production. The growth conditions ...

Aqueous ammonium ion battery is a promising sustainable energy storage system. However, the side reactions originating from electrolytes (the water decomposition and host material dissolution) preclude its practical applications. Unlike metal-based aqueous batteries, idea of “ultrahigh concentrated electrolyte” not feasible due to strong hydrolysis ions. Therefore, we propose an effective strat...

Poly(7-glutamic a id) (PGA) production ill Bacillas suhtitis IF03335 was studied. When citric acid as a carbon source was added to a glutamic acid medium containing L-glutamic acid and ammonium sulfate, a large amount of pure PGA wEs produced. On the other hand, when glucose was added to the glutamic acid medium, a by-preduct was produced, which seemed to be a polysaccharide. Moreoyer, the mode...