Membrane associated alpha-glucosidase activity was investigated in a methanogenic bioreactor (MR) and a biosulfidogenic bioreactor (SR). Temperature and pH optima studies showed temperature optima of 50 degrees C and pH optima of 8.0 for the alpha-glucosidases from both the MR and SR. Sulfide (at a concentration of 150 mg l(-1)) resulted in the complete loss of all alpha-glucosidase activity in...

This investigation reports the biochemical findings in a native-born Briton suffering from the adult form of fucosidosis. Alpha-L-fucosidase, alpha-L-iduronidase, and beta-D-galactosidase were studied in cultured fibroblast and leucocytes from the patient with fucosidosis, her maternal grandfather, and several normal controls. A complete lack of alpha-L-fucosidase activity was found in the pati...

Abstract We investigated the effects of substrate (cellulose or starch) and different clay contents on production microbial extracellular polymeric substances (EPS) concomitant development stable soil aggregates. Soils were incubated with amounts montmorillonite (+ 0.1%, + 1%, 10%) both without two substrates contrasting quality (starch cellulose). Microbial respiration (CO 2 ), biomass carbon ...

introduction : albumin, one of the most important plasma proteins, has a difficult process of synthesis and production. we compared two different methods for albumin purification: carboxymethyl cellulose (cm cellulose) resin exchange and diethylaminoethyl cellulose (deae cellulose) resin exchange in order to determine which resin could be more beneficial. materials and methods : two ion exchang...

Sialidase has been purified from rat liver cytosol 83,000-fold by sequential chromatography on DEAE-cellulose, CM-cellulose, Blue-Sepharose, Sephadex G-200, and heparin-Sepharose. When subjected to sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis, the purified cytosolic sialidase moved as a single protein band with Mr = 43,000, a value similar to that obtained by sucrose density g...

Type I procollagen secreted by matrix-free cells from chick embryo tendons was purified by DEAE-cellulose chromatography. Electron microscopy of segment-long-spacing aggregates of the procollagen demonstrated the presence of both NH2-terminal and COOH-terminal extensions not found in collagen. The procollagen was digested with bacterial collagenase and the COOH-terminal fragments were isolated ...

The purification of human C8 in milligram quantities from outdated human serum was achieved by ammonium sulfate precipitation (37.5-50% saturation) and ion exchange column chromatography employing CM-32 cellulose and QAE-Sephadex. The yield of C8 activity ranged from 2-9%, and the average purification was 1,700-fold. Fully reduced C8 was shown by SDS polyacrylamide gel electrophoresis to have t...

This study assesses the possibility of obtaining regenerated cellulose products (beads and films) from eucalyptus sawdust dissolving pulps produced by non-conventional processes, compared with a commercial pulp as reference. Eucalyptus were obtained soda pulping followed two TCF sequential bleaching processes OOpZ, OOp, cold extraction. The characterization involved alpha-, beta- gamma-cellulos...

there are many reports on the application of different hydrocolloids in the production of leavened breads; most of which show positive effects on bread quality. however, there is little information to prove such effect on flat breads. the main aim of this study was to improve the quality of barbari dough and bread (iranian flat bread) in terms of texture, taste and general acceptability of fres...

Measurement of gross alpha, gross beta activities and cosmogenic beryllium-7 (7Be) concentrations were made both daily and weekly during the years 2001--2004 from samples of atmospheric aerosols filtered from the air at Tehran Nuclear Research Center (35 41' N) and Zahedan (28 29' N). Weekly aerosol samples collected with the high-volume air samplers on cellulose filters were used to determine ...