A 59-year-old female who was on dialysis due to diabetic nephropathy was referred to our hospital for severe hyperparathyroidism refractory to intravenous vitamin D receptor activator treatment. With subsequent cinacalcet hydrochloride treatment, parathyroid hormone (PTH) levels were only slightly suppressed. However, progressive increases were observed in serum alkaline phosphatase (ALP) and b...

We have prepared murine monoclonal antibodies against isolated human bone alkaline phosphatase (ALP, EC 3.1.3.1). Hybridoma supernates were separately screened for reactivity against both human liver and bone ALP. Although most antibody-positive hybrids showed similar reactivity against both isoenzymes, one hybridoma produced an antibody that interacted preferentially with liver ALP. This antib...

We have determined the effect of diabetes mellitus and starvation/re-feeding on serum alkaline phosphatase (ALP) isoenzymes in the rat. Both ketotic (DKA) and nonketotic (NKDM) diabetic rats showed elevated total ALP activity, primarily due to an increase in intestinal and bone/liver ALP isoenzyme in the NKDM and DKA groups, respectively. There was a decrease in intestinal ALP activity in 3 day...

Abstract Alkaline phosphatase (ALP) is an important marker for many diseases, yet efficient and reliable detection method still lacking. Here, we present a novel photonic alginate hydrogel particle as ALP sensor based on the competitive combination of intermediary agent, pyrophosphate ion (PPi), with crosslinker, which can trigger phase-transition response from hydrogel. This be converted into ...

The effect of daily, oral administration of ethanolic extract of Khaya senegalensis stem bark (2mg/kg body weight) for 18days on the alkaline phosphatase, aspartate and alanine aminotransferase activities of rat liver and serum were investigated. Compared with the control, the activities of liver alkaline phosphatase (ALP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) inc...

The activity of mouse endometrial alkaline phosphatase after hyperstimulation and progesterone injection at the implantation time Alkaline phosphatase (ALP) of endometrium may play a critical function in the development and implantation of embryo. The aim of this study was to determine the localization of endometrial ALP activity after hyperstimulation and progesterone injection. Thirty adult f...

With the aim to detect candidate malignant primitive progenitor populations, we modified an original alkaline phosphatase (ALP) stem cell detection method based on the identification of alkaline phosphatase fluorescent cells in combination with flow cytometry immunophenotyping. Over a period of one year, we have been using this technique to study its activity in patients with leukemia and lymph...

Alkaline phosphatase (ALP) activities were compared in fibroblasts from three cystic fibrosis patients and two normal controls after culturing the cells in normal growth medium and in medium containing Tamm-Horsfall glycoprotein, isoproterenol, and theophylline. No consistent alterations in ALP activities were noted, either between the same cell lines grown under different conditions, or betwee...

introduction: the aim of this study was to evaluate the correlation between serum levels of ovarian and gonadotropin hormones, age and number of follicles with follicular alkaline and acid phosphatase activity in infertile women under controlled ovarian hyperstimulation. methods: after collection of follicular fluid and calculation of the number of follicles, the specific activity of alkaline (...