Crystallization of a family 9 beta-1,4-glucanase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius CelA is reported. Thin plates can be obtained by hanging-drop vapour-diffusion crystallization in high concentrations (60%) of MPD. These crystals are unusual in that they do not bind the dye IZIT in the mother liquor and do not appear to dissolve in water after three weeks or i...

The Friedel-Crafts alkylation constitutes one of the most important reactions for formation carbon-carbon bonds. Here, we report using squalene-hopene cyclases, which provides a biological alternative to traditional strategy. cyclase from Alicyclobacillus acidocaldarius (AacSHC) and variants have been demonstrated broad substrate reaction scope, making them valuable potential applications in bi...

N-acetyl glutamate synthase (NAGS) is the first enzyme in arginine biosynthetic pathway and catalyzes transfer of an acetyl group from acetyl-CoA to L-glutamate produce N-acetylglutamate (NAG). NAGS enzymes either consist two domains (classical NAGS) or a single domain (short S-NAGS). S-NAGS, although present many bacteria, have not been well characterized date. This study's focus kinetically c...