GRB-PAP1 is a continuous line of epithelial cells derived from a rabbit renal inner medulla. Elevation of the NaCl concentration in the medium bathing these cells strongly induced the expression of a soluble protein with an apparent molecular mass of 39 kDa. The protein, purified by affinity chromatography with Amicon Matrex Gel Orange A, had enzyme activity characteristic of aldose reductase (...

The substrate specificities of human aldose reductase and aldehyde reductase toward trioses, triose phosphates, and related three-carbon aldehydes and ketones were evaluated. Both enzymes are able to catalyze the NADPH-dependent reduction of all of the substrates used. Aldose reductase shows more discrimination among substrates than does aldehyde reductase and is generally the more efficient ca...

The effects of some antibiotics and metals on goat (Capra aegagrus hircus) kidney aldose reductase (AR) sorbitol dehydrogenase (SDH) activities were examined. For drugs that exhibit inhibitory effect, Ki constants calculated inhibition types determined by using Lineweaver-Burk curves. In our study, the highest showed ceftriaxone antibiotic in AR enzyme with an IC50 value 0.0274 mM. More potent ...

Review of literature shows that large varieties of compounds have been found to bear hypoglycemic action. For instance, glycosides afford active principles that lower blood sugar in test animals. Following the I.V. administration of a 4 percent decoction of the dried T. polium herb, a definite reduction of blood sugar level has been exhibited. With reference to PDR for Herbal Medicines for the ...

Abnormalities in corneal epithelial healing in diabetic patients have been described recently. Defects in corneal re-epithelialization in diabetic rats have been reported, and it was found that treatment with aldose reductase (AR) inhibitors effectively prevented these defects. Experiments using galactosemic rats to study further the role of AR in these defects, since AR is known to be the comm...

During antidiuresis, renal papillary cells accumulate organic osmolytes preferentially over inorganic ions. It has been previously demonstrated that sodium infusion increased all of these organic osmolytes except myo-inositol (1). Conversely, urea infusion increased only glycerophosphorylcholine significantly. In addition to sodium and urea, potassium localized in tissue and urine influenced th...

background: the relationship between the high activity of aldose reductase (ar) and diabetic cataract formation has been previously investigated. the purpose of the present study was to determine the preventing effect of melatonin on streptozotocin (stz)-induced diabetic cataract in rats. methods: 34 adult healthy male sprague-dawely rats were divided into four groups. diabetic control and diab...

In vitro antidiabetic effect of fruits and vegetables with reports as folk remedies were investigated. The antidiabetic effects were evaluated by comparing the inhibitory properties of α-glycosidase, aldose reductase, and antioxidant activity. The results indicated that lychee extract exhibited the best dose-dependent inhibitory activity against α-glycosidase with IC 50 of 10.4 mg/mL, and lemon...