SERUM ALDOLASE ESTIMATION 1S one of the principal diagnostic tests used in the elucidation of muscle disease (2-6). In myocardial infarction (7), liver disease (8), and carcinomatosis (9), the serum aldolase estimation is a useful ancillary aid to diagnosis. The usefulness of the test has been enhanced since the differentiation 1)etween muscle and liver aldolase by use of two substrates-fructos...

1. Aldolase was selected as a suitable marker for following the androgenic regulation of mRNA synthesis in the prostate gland. 2. Antibodies raised in rabbits against crystalline prostate aldolase were used to monitor the synthesis of this androgen-induced enzyme after hormonal stimulation of castrated animals, by using procedures in vivo and in vitro for the translation of prostate poly(A)-ric...

The products of reactions catalyzed by Methanococcus. jannaschii (Mj) aldolase using various substrates were identified by gas chromatography (GC). Although Mj aldolase is considered a fuculose-1-phosphate aldolase based on homology searching after gene sequencing, it has not been proven to be a fuculose-1-phosphate aldolase based on its reaction products. Mj aldolase was found to catalyze reac...

The effects of inositol 1,4,5-trisphosphate (Ins(1,4,5)P3), which has been hypothesized to be a chemical transmitter in excitation-contraction coupling in skeletal muscle, on aldolase bound to isolated triad junctions were investigated. Fructose-1,6-bisphosphate aldolase was identified as the major specific binding protein for the Ins(1,4,5)P3 analogue glycolaldehyde (2)-1-phospho-D-myo-inosito...

The messenger activity for fructose 1,6-bisphosphate aldolase (EC4.1.2.13) (aldolase) A isozyme has been characterized in the polysome- or the messenger RNA-directed, protein-synthesizing system using the pH 5 fraction of rat liver or wheat germ extracts, respectively. The subunit of aldolase A synthesized in vitro was detected by immunoprecipitation with anti-aldolase A antibody raised in chic...

In cancer, glucose uptake and glycolysis are increased regardless of the oxygen concentration in the cell, a phenomenon known as the Warburg effect. Several (but not all) glycolytic enzymes have been investigated as potential therapeutic targets for cancer treatment using RNAi. Here, four previously untargeted glycolytic enzymes, aldolase A, glyceraldehyde 3-phosphate dehydrogenase, triose phos...

Aldolase was isolated in a crystalline form in a 6% yield from rat Rhodamine sarcomas. The purification involved extraction with Tris-buffer, batchwise treatment with diethylaminoethylcellulose, ammonium sulfate fractionation, column chromatography on cellulose phosphate, and crystallization from ammonium sulfate solution (0.35 saturation). The crystalline aldolase preparation behaved as a homo...

Mast cells (MC) are primary effector cells of IgEmediated allergic inflammation. Nitric oxide (NO) is a short-lived free radical that regulates MC activities including inhibition of MC degranulation. To elucidate the molecular mechanisms underlying the effects of NO in MC, we investigated protein tyrosine nitration in human mast cell lines treated with the NO donor SNitrosoglutathione (SNOG). U...

Aldolase was isolated in a crystalline form in a 6% yield from rat Rhodamine sarcomas. The purification involved extraction with Tris-buffer, batchwise treatment with diethylaminoethylcellulose, ammonium sulfate fractionation, column chromatography on cellulose phosphate, and crystallization from ammonium sulfate solution (0.35 saturation). The crystalline aldolase preparation behaved as a homo...