BACKGROUND Turf soil bacterial isolate Delftia sp. VM4 can degrade exogenous N-acyl homoserine lactone (AHL), hence it effectively attenuates the virulence of bacterial soft rot pathogen Pectobacterium carotovorum subsp. carotovorum strain BR1 (Pcc BR1) as a consequence of quorum sensing inhibition. METHODOLOGY/PRINCIPAL FINDINGS Isolated Delftia sp. VM4 can grow in minimal medium supplemente...

Adhesive high-level replacement (HLR) systems have been recently established as a suitable categorical framework for double pushout transformations based on weak adhesive HLR categories. Among different types of graphs and graph-like structures, various kinds of Petri nets and algebraic high-level (AHL) nets are interesting instantiations of adhesive HLR systems. AHL nets combine algebraic spec...

Adhesive high-level replacement (HLR) systems have been recently established as a suitable categorical framework for double pushout transformations based on weak adhesive HLR categories. Among different types of graphs and graph-like structures, various kinds of Petri nets and algebraic high-level (AHL) nets are interesting instantiations of adhesive HLR systems. AHL nets combine algebraic spec...

The chemolithoautotrophic bacterium Nitrosospira multiformis is involved in affecting the process of nitrogen cycling. Here we report the existence and characterization of a functional quorum sensing signal synthase in N. multiformis. One gene (nmuI) playing a role in generating a protein with high levels of similarity to N-acyl homoserine lactone (AHL) synthase protein families was identified....

Bacterial populations coexisting in the phyllosphere niche have important effects on plant health. Quorum sensing (QS) allows bacteria to communicate via diffusible signal molecules, but QS-dependent behaviors in phyllosphere bacterial populations are poorly understood. We investigate the dense and diverse N-acyl-homoserine lactone (AHL)-producing phyllosphere bacteria living on tobacco leaf su...

Given that a large proportion of the bacteria colonizing the roots of plants is capable of producing N-acyl-L-homoserine lactone (AHL) molecules, it appears likely that these bacterial pheromones may serve as signals for communication between cells of different species. In this study, we have developed and characterized novel Gfp-based monitor strains that allow in situ visualization of AHL-med...

Algebraic High-Level (AHL) nets are a well-known modelling technique based on Petri nets with algebraic data types, which allows to model the communication structure and the data flow within one modelling framework. Transformations of AHL-nets – inspired by the theory of graph transformations – allow in addition to modify the communication structure. Moreover, high-level processes of AHL-nets c...

Serum paraoxonases (PONs) are calcium-dependent lactonases that catalyze the hydrolysis and formation of a variety of lactones, with a clear preference for lipophilic lactones. However, the lactonase mechanism of mammalian PON1, a high density lipoprotein-associated enzyme that is the most studied family member, remains unclear, and other family members have not been examined at all. We present...