Nanostructured surfaces enhance ion yields in matrix-assisted laser desorption–ionization mass spectrometry (MALDI-MS). The spike protein complex, S1, is one fingerprint signature of Sars-CoV-2 with a 75 kDa. Here, we show that MALDI-MS ions the 100 kDa range are enhanced 50-fold when matrix–analyte solution placed on substrates coated dense forest multi-walled carbon nanotubes, compared to fro...

 The Indian riverine buffaloes are more susceptible to variations in the environment. These variations affect seminal plasma proteins, which in turn affect fertility. This study was conducted to evaluate variations in the Indian Bubalus bubalis seminal plasma protein profile using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) during winter and summer seasons, and its relation wit...

The whitefish industry generates a huge amount of rest raw material, which is currently wasted or underutilized in the production low-value products such as animal feed. While fish muscle primary product use for human consumption, material has great potential source protein and bioactive peptides food ingredients nutraceuticals. Enzymatic hydrolysis biotechnological processing method that can b...

Typhoid fever is a multiorgan infectious disease caused by Salmonella typhi. It transmitted through fecal oral route and can be fatal without proper treatment. Therefore, early diagnosis of typhoid crucial. In the previous study, we have developed TYPHOIDYNE EIA, which showed excellent synergy between genus conserved species-specific antigens in serodiagnosis fever. EIA effectively detect diffe...

edwardsiella tarda is a versatile pathogen that could survive in different environmental conditions and infect economically important fish species. the whole cell antigenic proteins were extracted from e. tarda and assessed via western blot using anti e. trada rohu and anti e. trada rabbit serum. two strong reacted proteins with antibodies were recovered viz 61 kda and 47 kda from sds page gels...

background: hydatidosis is one of the most important helminthic diseases causing serious health and economic problems in many countries including iran. currently available diagnostic approaches for diagnosis of cystic echinococcosis (ce) are not satisfactory since they have not achieved a reasonable validity in diagnosis of ce. this study aimed to assess the perform¬ances of western blotting (w...

This study aimed to quantify the effect of membrane surface porosity on particulate fouling predicted by MFI-UF method at constant flux. Firstly, polyethersulfone UF membranes (5–100 kDa) was determined using ultra-high resolution SEM. Thereafter, measured suspensions polystyrene particles (75 nm), which were pre-washed remove surfactant and particle fractions smaller than pores membranes, thus...