Some calcium antagonists and calmodulin inhibitors enhance the intracellular levels of vincristine and Adriamycin in vincristine- and Adriamycin-resistant P388 leukemia cells by inhibiting their outward transport. The high intracellular drug accumulation was directly related to the enhancement of the cytotoxicity of the antitumor agents, and the vincristine and Adriamycin resistance in these ce...

Adriamycin has a wide variety of biological actions on susceptible cells, several of which may be integrally involved in cytotoxicity. In this paper, we present evidence that one of the alterations in cell function that occurs in the presence of Adriamycin is an elevation in the production of diacylglycerol. The effect is rapid, reaches a peak within 10 min of exposure of Sarcoma 180 cells to A...

abstract background: chemotherapy- induced nausea and vomiting (cinv) occur frequently causing problems with an unacceptably high incidence that significantly affect patients' daily functioning and health-related quality of life. the present study was aimed to compare acute cinv for granisetron as 5-ht3 receptor antagonist and metoclopramide in the patients receiving chemotherapeutic regimens i...

Exponentially growing Chinese hamster V79 cells were exposed to Adriamycin either in phosphate buffered saline (PBS) or fresh growth medium (F-med) supplemented with various amounts of NaCl in the range between 50-1000 mM and survival was measured by the colony forming assay. Compared to the survival obtained after exposure of cells to isotonic (140 mM NaCl) PBS (D0 = 0.16 microgram/ml, Dq = 0....

The effect of caffeine, at concentrations between 10 microM and 20 mM was studied on Adriamycin-induced cytotoxicity and DNA damage in exponentially growing Chinese hamster V79 cells. Simultaneous administration of caffeine with 0.4 micrograms/ml (0.69 microM) Adriamycin for 1 h resulted in a concentration-dependent reduction in cell killing. The surviving fraction increased from 0.001 for cell...

Multidrug resistance-associated protein 1 (MRP1) mediates the ATP-dependent efflux of endobiotics and xenobiotics, including estradiol 17-(beta-d-glucuronide), leukotriene C(4), and the reduced glutathione conjugate of 4-hydroxy-2-nonenal (HNE), a highly reactive product of lipid peroxidation. Adriamycin is an effective cancer chemotherapeutic drug whose use is limited by cardiotoxicity. Adriam...

PURPOSE Current combination treatment strategies in malignancy are designed to evaluate the use of cytotoxic drugs and antiangiogenic agents. Endostatin, a fragment of collagen XVIII, specifically inhibits proliferation, migration, and differentiation of endothelial cells in vitro as well as angiogenesis and tumor progression in in vivo models. In this study, we determine the antitumor effect o...

7-con-O-Methylnogarol (7-OMEN) is a new anthracycline antitumor agent with significant activity in vivo against murine P388 and L1210 leukemia (140% increase in life span) and B16 melanoma (114% increase in life span). We report here the complex relationship that exists between intracellular drug concentration and killing of Chinese hamster ovary (CHO), B16, and L1210 cells in culture by 7-OMEN...