Tetrazolium-dye-linked alcohol dehydrogenase (TD-ADH) of Amycolatopsis methanolica could be resolved into three protein components, which have been purified. Each of the components has the ability to reconstitute TD-ADH activity when combined with the other two. Component 1 is identical to the previously characterized methanol:N,N'-dimethyl-4-nitrosoaniline oxidoreductase (MNO), a decameric pro...

We read with a great pleasure the article ‘Rare ADH variant constellations are specific for alcohol dependence’ by Zuo et al. (2012). We want to direct the speciality about the alcohol dehydrogenase (ADH) isoenzyme nomenclature to the readers of Alcohol and Alcoholism. Namely, the authors use an old isoenzyme nomenclature what can cause some confusion to not enough familiar readers. Electrophor...

This study deals with biochemical and metabolic-physiological aspects of the relationship between variation in in vivo alcohol dehydrogenase activity and fitness in larvae homozygous for the alleles Adh71k, AdhF, AdhS, of Drosophila melanogaster, and for the common Adh allele of Drosophila simulans. The Adh genotypes differ in the maximum oxidation rates of propan-2-ol into acetone in vivo. The...

Abstract Purpose This work studies the immobilization of two enzymes, alcohol dehydrogenase (ADH) and aldehyde (AldDH) both from Saccharomyces cerevisiae , which could be used to produce high value-added molecules carboxylic acids embedded in anaerobic digestate. Methods In particular, three mesoporous siliceous materials, with different specific surface areas pore sizes, (MSU-H, MSU-F MCF 0.75...

The enzyme activity of alcohol dehydrogenases 4W6Z and 1MG5 demonstrated good agreement with the calculated a-carbon atomic partial charges of the substrates. The correlation coefficients were found to be more than 0.8 (n = 9) for both the oxidation and reduction reactions. The stereo structure of cinnamylalcohol dehydrogenase (ELI3-2) was constructed modification in 1PIW (cinnamyl alcohol dehy...

Alcohol dehydrogenase (Adh) (alcohol:NAD+ oxidoreductase, EC 1.1.1.1) gene frequencies and ethanol tolerance in Drosophila melanogaster are known to exhibit long-range latitudinal variations on different continents; this has led to the argument that the clines are adaptive. Accordingly, tropical populations are characterized both by a low frequency of Adh-F and by a low ethanol tolerance. In th...

The effects of thyroid hormone on hepatic and gastric alcohol dehydrogenase (ADH) activities (nM of NADH/min/mg of cytosolic protein) have been investigated in male Sprague Dawley rats treated with thyroxine (1 mg/kg, po) for 14 days. Whereas hepatic ADH activity in thyroxine-treated rats decreased by 61.3% of control rats (26.4 vs 43.2, p<0.001), gastric ADH activity increased by 262.9% of con...

We describe the characterization of a previously reported control mutation, AdhSL, in the alcohol dehydrogenase gene of Drosophila melanogaster, which results in decreased production of ADH molecules and subsequently lower ADH activity in adults. We find that the regulatory element modifies ADH mRNA levels and acts cis on both ADH protein and mRNA. It is not promoter specific but is development...

Two unlinked genes which specify alcohol dehydrogenase (ADH) enzymes in maize are coordinately regulated by competition for a factor which limits the rate of total ADH expression during anaerobiosis. The "gene competition hypothesis" and the existence of organ-specific competitive programs, as proposed by Schwartz (1971), is further supported. The potential balance of expression between these t...

Alcohol dehydrogenase (E.C 1.1.1.1) is an enzyme that catalyses the reduction of acetaldehyde to ethanol during fermentation. In this study, activity crude alcohol (ADH) isolated from yeast (Saccharomyces cerevisiae) fresh Raphia hookeri and Elaeis guineensis palm wine(saps) Niger Delta region (Akwa Ibom, Bayelsa, Cross River, Delta, Edo, Imo River States) was determined at a temperature range ...