TAF 4b was first discovered as a tissue-specific subunit of TFIID in human B cells. The development of infertility in TAF 4b knockdown mice revealed its role in gonadal development. Its knockdown results in an increase in FSHβ expression, and affects the expression of several regulatory proteins involved in the FSHβ regulation in the LβT2 gonadotrope cell line. However, TAF 4b is not associated...

The RNA polymerase II general transcription factor TFIID is a complex containing the TATA-binding protein (TBP) and associated factors (TAFs). We have used a mutant allele of the gene encoding yeast TAF(II)68/61p to analyze its function in vivo. We provide biochemical and genetic evidence that the C-terminal alpha-helix of TAF(II)68/61p is required for its direct interaction with TBP, the stabl...

PURPOSE To compare the safety and efficacy of fixed-dose tafluprost/timolol combination (Taf/T-FDC) with those of fixed-dose latanoprost/timolol combination (Lat/T-FDC) by measuring the intraocular pressure (IOP)-lowering effect, ocular pharmacokinetics, and ocular surface toxicity. METHODS The IOP-lowering effect of Taf/T-FDC and Lat/T-FDC in ocular normotensive monkeys was evaluated at 4 an...

Determination of the crystal structure of the human TBP-associated factor (hTAF(II))28/hTAF(II)18 heterodimer shows that these TAF(II)s form a novel histone-like pair in the TFIID complex. The histone folds in hTAF(II)28 and hTAF(II)18 were not predicted from their primary sequence, indicating that these TAF(II)s define a novel family of atypical histone fold sequences. The TAF(II)18 and TAF(II...

Background: Recent evidence suggests that integrase strand transfer inhibitors are associated with greater weight gain than protease in patients human immunodeficiency virus (HIV-1). Objectives: To describe demographic and clinical characteristics of insured HIV-1 the United States initiating darunavir/?cobicistat/?emtricitabine/?tenofovir alafenamide (DRV/c/FTC/TAF) or bictegravir/FTC/TAF (BIC...

Tumour angiogenesis is the process whereby a capillary network is formed from a pre-existing vasculature in response to tumour secreted growth factors (TAF). The capillary network is largely composed of migrating endothelial cells which organise themselves into dendritic structures. In this paper we model angiogenesis via the theory of reinforced random walks, whereby the chemotactic response o...

We study the effects of the U.S. Federal Reserve’s Term Auction Facility (TAF) on globally active banks. We exploit a unique dataset with information on all foreign activities of German banks’ affiliates both, inand outside the US. All German parent banks with US affiliates tapped TAF liquidity, which mitigates self-selection concerns. This setting allows testing whether foreign affiliate activ...

The aim of the study was to investigate the possibility of improving the therapeutic efficacy of the total alkaloid fraction (TAF) extracted from processed nux vomica by reducing the strychnine contents. Most strychnine was removed from TAF to obtain the modified total alkaloid fraction (MTAF). The toxicity and pharmacokinetics of TAF and MTAF were further investigated and compared besides thei...

The general transcription factor TFIID is composed of the TATA box binding protein (TBP) and multiple TBP-associated factors (TAFs). In yeast, promoters can be grouped into two classes based on the involvement of TAFs. TAF-dependent (TAF(dep)) promoters require TAFs for transcription, and TBP and TAFs are present at comparable levels on these promoters. TAF-independent (TAF(ind)) promoters do n...

Independent security functional testing on a product occupies a backseat in traditional security evaluation because of the cost and stringent coverage requirements. In this paper we present the details of an approach we have developed to automate security functional testing. The underlying framework is called TAF (Test Automation Framework) and the toolkit we have developed based on TAF is the ...