This study deals with total phenolic content, antiproliferative and proapoptotic activity of methanolic extracts from different Teucrium species and the effect on the prooxidant/antioxidant status in HCT-116 cells. The total phenolic content of the extracts was measured spectrophotometricaly and the obtained results ranged from 56.62 mg/g to 172.50 mg GA/g. The antiproliferative activity of met...

Abstract Cell viability is one of the critical quality attributes (CQAs) for analyzing and characterizing cellular therapeutic products. The characterization cell essential to ensure quality, safety efficacy during gene therapy development. In this work, we characterized compared two commonly used dual-staining methods, acridine orange/propidium iodide (AO/PI) orange/4′,6-diamidino-2-phenylindo...

BACKGROUND India ink microscopy on cerebrospinal fluid is still utilized in resource limited settings for the diagnosis of cryptococcal meningitis despite its poor sensitivity. We hypothesized that staining fungal nucleic acids with fluorescent dyes instead of the capsule with India ink might improve sensitivity for the diagnosis of cryptococcal meningitis. METHODS We enrolled 96 HIV-infected...

In order to easily assess growth and destruction of Toxoplasma gondii in vitro, this report describes two double staining assays that both visualize live and dead organisms: acridine orange--ethidium bromide (AO-EB) and bisbenzimide (Hoechst 33258)--propidium iodide (B-PI). EB and PI were chosen for dead organisms staining while AO and B stain viable organisms. Thus, both double staining assays...

A new fluorescent acridine orange derivative, 3-amino-6-methoxy-9-(2-hydroxyethylamino) acridine (AMHA), has been applied to Hela cells in order to set up appropriate conditions for the detection of mycoplasma contaminations. Since AMHA staining reveals intensely fluorescent nuclei and slight fluorescent cytoplasm, we can visualize and localize mycoplasma contamination on each cell. In combinat...

1-(Acridin-9-yl)-3-disubstituted thioureas react with methyl bromoacetate and bromoacetonitrile, respectively, depending on a substituent bulkiness, to polyfunctionally substituted (acridin9-yl)imino-1,3-thiazolidin-4-ones and spiro[9,10-dihydroacridine-9,4-thiazolidines]. Reactions represent the simple and convenient way to synthesize the title compounds with possible antibacterial activity. B...

brucellosis is one of the most common zoonotic diseases in iran and human brucellosis is endemic in all parts of the country. growth of brucella is slow and blood culture of these bacteria by use of classical methods is time-consuming. furthermore, in endemic area culture is required for definitive diagnosis. in the present study, direct urease test and acridine orange staining were tried on th...