A 58-day-old female infant reported with complaints of fever, difficult breathing, loose stool, vomiting and refusal to feed for 4 days. Laboratory work showed anaemia, leucocytosis elevated neutrophils thrombocytopenia along high C-reactive protein D-dimer bilateral patchy infiltrate on X-ray positivity COVID-19. Her blood culture was also positive Gram-negative bacilli (acinetobacter lwoffii)...

BACKGROUND & OBJECTIVE The production of carbapenemases is an important mechanism responsible for the carbapenem resistance. A simple and inexpensive testing method for screening of carbapenemase producers is essential. A prospective study was undertaken to detect metallo-beta-lactamases (MBLs) and AmpC beta-lactamases in nonfermentative Gram negative bacteria and to evaluate the various method...

Genomic species diversity among 147 Acinetobacter clinical isolates not belonging to the A. calcoaceticus- A. baumannii (ACB) complex was investigated by phenotypic and genotypic identification methods. The isolates were obtained between 1991 and 1999 from numerous diagnostic laboratories in the Czech Republic and were studied by numerical probabilistic identification using two biochemical freq...

A microarray consisting of oligonucleotide probes targeting variable regions of the 16S rRNA gene was designed and tested for the investigation of microbial communities in compost. Probes were designed for microorganisms that have been previously reported in the composting process and for plant, animal and human pathogens. The oligonucleotide probes were between 17 and 25 bp in length and inclu...

Nonmammalian model systems of infection such as Galleria mellonella (caterpillars of the greater wax moth) have significant logistical and ethical advantages over mammalian models. In this study, we utilize G. mellonella caterpillars to study host-pathogen interactions with the gram-negative organism Acinetobacter baumannii and determine the utility of this infection model to study antibacteria...

A cluster (27 kbp) of genes responsible for the biosynthesis of the amphipathic, polysaccharide bioemulsifier emulsan from the oil-degrading Acinetobacter lwoffii RAG-1 was isolated and characterized. The complete sequence of this cluster, termed wee, consisted of 20 ORFs. One set of 17 ORFs was transcribed in one direction, while a second set of three ORFs, 607 bp upstream of the first, was tr...

ABSTRACT Endophytic bacteria with multifunctional characteristics can benefit plants through different mechanisms, as well promoting growth in an efficient, low-cost and ecofriendly way. This study analyzed the potential of endophytic isolate Acinetobacter lwoffii Bac109 early vitro sugarcane seedlings. The strain showed to solubilize phosphate a solid medium (solubilization index: 3.73). In ad...