This study aimed to examine the prebiotic effect of Carao (Cassia grandis) pulp powder on probiotic characteristics Lactobacillus acidophilus regarding viability, enzymatic activity, lysozyme resistance, bile and acid tolerances, tolerance gastric juices. was used at 0% (control), 1%, 2%, 3% (w/v). Acid were determined 0, 30, 60, 90, 120 min incubation, whereas analyzed 4, 8 h. The juice pH 2, ...

Lactobacillus rhamnosus GG is a lactic acid bacterium that is widely used as probiotic products in the dairy industry. To gain insights into the genome stability of the L. rhamnosus GG in the human gastrointestinal tract and the possible adaption mechanism under different stresses, we first examined the genotype and phenotype of the L. rhamnosus GG grown over 1000 generations under various stre...

Commensal and pathogenic microorganisms must resist the deleterious actions of bile in order to survive in the human gastrointestinal tract. Herein we review the current knowledge on the mechanisms by which Gram-positive and Gram-negative bacteria contend with bile stress. We describe the antimicrobial actions of bile, assess the variations in bile tolerance between bacterial genera and examine...

conclusions these human-derived lab and bifidobacteria exhibited different mechanisms involved in pathogenic inhibition. therefore a combination of these probiotic strains could be a great promise and possibility for the development of probiotic products to effectively prevent and control food-borne infection in humans. background food-borne pathogens are among the most significant problems in ...

Objective Intrahepatic cholestasis of pregnancy (ICP) is a rare liver disorder, usually manifesting in the third trimester and associated with increased perinatal morbidity and mortality. The hallmark laboratory abnormality in ICP is elevated fasting serum bile acids; however, there are limited data on whether a nonfasting state affects a pregnant woman's total bile acids. This study assesses f...

Lactobacillus acidophilus 30SC has been isolated from swine intestines and considered a probiotic strain for dairy products because of its ability to assimilate cholesterol and produce bacteriocins. Here, we report the complete genome sequence of Lactobacillus acidophilus 30SC (2,078,001 bp) exhibiting strong acid resistance and enhanced bile tolerance.

CONTEXT Besides their role in intestinal resorption of lipids, bile acids are regarded as endocrine and metabolic signaling molecules. The detailed profile of bile acid species in peripheral blood after an oral lipid tolerance test (OLTT) is unknown. OBJECTIVE We quantified the regulation of 18 bile acids after OLTT in healthy individuals. MATERIAL AND METHODS 100 volunteers were characteri...