Geissoschizine methyl ether (1), a newly discovered strong acetylcholinesterase (AChE) inhibitor, along with six weakly active alkaloids, vallesiachotamine (2), hisuteine (3), hirsutine (4), isorhynchophylline (5), cisocorynoxeine (6) and corynoxeine (7) have been isolated from Uncaria rhynchophylla. Geissoschizine methyl ether (1) inhibited 50% of AChE activity at concentrations of 3.7 ± 0.3 µ...

Acetylcholinesterase (AChE) levels were determined in control samples of cat cerebral cortex, in partially neuronally isolated (undercut) cortex, and in undercut or intact cortex that had received long-term intermittent electrical stimulation. Assay of AChE activity was based on an automatic titration method using methalcholine as substrate. Tissues from ten of 15 undercut cerebral cortices sho...

Alzheimer's disease (AD) and diabetes mellitus (DM) are related to abnormal changes in enzyme activity. While acetylcholinesterase (AChE) butyrylcholinesterase (BChE) the primary targets treatment of (AD), α-glucosidase (α-Gly) α-amylase (α-Amy) enzymes known for (DM). Here, benzenesulfonamide-based thiourea thiazolidinone derivatives such as AChE, BChE, α-Gly, α-Amy inhibitors were reported. T...

A sensitive and flexible detection method for organophosphorus pesticides (OPs) is a crucial request to avoid their further expanded pollution. Herein, an acetylcholinesterase (AChE) electrochemical sensor, based on the co-modification of ZIF-8 graphene (GR), was constructed OPs. ZIF-8/GR composite can provide stable biocompatible environment loading AChE accelerate chemical reaction electrode ...

Although a recent study (Zhang et al. Cell Death Differ 2002; 9; 790-800) presented that acetylcholinesterase (AChE) might be an important common component in leading to various types of apoptosis, the molecular mechanism, by which AChE functions, had remained elusive before that study. We explored the role of AChE in apoptosis by silencing the AChE gene. Silencing of the AChE gene abolished th...

Owing to the threat of organophosphate exposures, not only to pesticides but also to nerve agents, it is very important to know the whole process of organophosphates-inhibited acetylcholinesterase (AChE, EC 3.1.1.7) reactivation. Although current antidotes against organophosphorus intoxications consist also of prophylactics, AChE reactivators are still needed especially in the case of intoxicat...

Autoimmune myasthenia gravis (MG) is a neuromuscular disorder caused by autoantibodies directed against the acetylcholine receptor (AChR). Current symptomatic therapy is based on acetylcholinesterase (AChE) drugs. The available long-term current therapy includes steroids and other immunomodulatory agents. MG is associated with the production of a soluble, rare isoform of AChE, also referred as ...

Preparation of 1-(4-hydroxy-iminomethylpyridinium)-3-pyridiniumpropane dibromide is described. This compound represents a new acetylcholinesterase (AChE) reactivator, which has no substituents on the second pyridinium ring as found in other commonly used AChE reactivators. The reactivation ability of this reactivator was tested on tabun- and cyclosarin-inhibited AChE. According to the results o...

Primary nerve cell cultures contain acetylcholinesterase (AChE: EC 3 .l .1.7) and other special macromolecules associated with cholinergic transmission [l--4 J . Cultured chick nerve cells, like cultured muscle, have been shown to release AChE into their medium [ 5 $1. The experiments presented here examined the levels of activity and release of AChE in nerve cell cultures after treatment with ...