Mapping epigenetic modifications and identifying their roles in the regulation of spermatogenesis and embryogenesis are essential for gaining fundamental medical understandings and for clinical applications. More and more evidence has shown that specific epigenetic modifications are established during spermatogenesis, which will be transferred into oocyte via fertilisation, and play an importan...

Abnormal embryo cortisol level causes developmental defects and poor survival in zebrafish (Danio rerio). However, no study has demonstrated that maternal stress leads to higher embryo cortisol content in zebrafish. We tested the hypothesis that maternal stress-associated elevation in cortisol levels increases embryo cortisol content in this asynchronous breeder. Zebrafish mothers were fed cort...

BACKGROUND Sperm chromatin status and nuclear DNA damage can be detected using well-established assays. However, most techniques are time-consuming and/or involve elaborate protocols and equipment. We have recently developed a simple and fast method to monitor sperm chromatin status in field conditions using the Diff-Quik assay which is employed in fertility clinics to assess sperm morphology w...

Bull effect on results of in vitro embryo production has been well documented. The aim of the present study was to find the relationship between quality of bull sperm chromatin and its effect on in vitro embryo production. Bovine in vitro matured oocytes were fertilized in vitro using capacitated spermatozoa (freshly ejaculated or frozen-thawed) of 12 bulls. Semen was simultaneously processed a...

In this study, we investigate developmental patterns of body movements that may play roles in normal embryonic growth using direct recordings chick embryos during the early stages development (72–125 h incubation). three individuals, occurred intermittently and irregularly after 72-h incubation, frequencies sizes increased with growth. Additionally, became periodic at 85 all cyclic periods were...

The aim of the study was to compare the single pregnancy and neonate outcome after fresh and frozen-thawed embryo transfer in the in vitro fertilization programme (IVF). The study focused on clinical and laboratory factors affecting the abnormal placentation, especially placenta praevia, in patients conceiving in the IVF programme. The results confirm that neonates born after frozen-thawed embr...

background: successful embryo transfer (et) technique is a fateful step of all efforts to achieve live births from in vitro produced embryos in assisted reproductive techniques or in knockout, transgenic or cloned animal projects. small reproductive tract of mice and limitation of current techniques may not well satisfy the requirements for mass production of genetically modified mice. genetic ...

Introduction The mouse embryo is a widespread animal model for human development disorders. MRI offers a unique, noninvasive approach for imaging normal and abnormal mouse embryo development in later gestational stages. One of the difficulties of imaging in utero mouse embryos is the lack of tissue contrast exhibited by the embryo at high magnet field strengths. This project explores the use of...

The many different types and variations of deformities of limbs in animals and man are usually described and grouped from the morphological or genetical point of view. A very few biological experiments have been done for the purpose of elucidating the causes which produce special types of deformities in the embryo. In an earlier paper (Brandt, 1940) the author has shown that supernumerary limbs...