Rapidly developing technologies such as quantitative real-time reverse transcriptase–polymerase chain reaction (RT-PCR) and microarrays enable characterization and quantification of nucleic acids in clinical samples. Identification of DNA from pathogens such as bacteria or viruses is already widely used in molecular diagnostics. 1 The use of RNA as a diagnostic tool is currently being investiga...

Confirmation of mutations mostly involves DNA sequencing. The conventional manual radioactive labeling sequencing protocol may be conceived as the method of choice because of the perception that the capital and operational costs of automated sequencers are high. Velickovic et al. (3) reduced the cost of DNA sequencing by running 48 or 64 samples on a 36-lane model 377-01 ABI PRISM automated se...

STR multiplexes have been indispensable for the efficient genotyping of forensic samples. The PowerPlex 16 System contains the coreCODIS loci, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, CSF1PO, FGA, THOI, TPOX, vWA, the sex determinant locus, amelogenin, and two pentanucleotide STR loci, Penta D and Penta E. This multiplex satisfies the locus requirements for most natio...

BACKGROUND The ankle-brachial index (ABI) is a noninvasive method to evaluate peripheral artery disease (PAD). ABI <0.9 diagnoses PAD; ABI >1.3 is a false negative caused by noncompressible arteries. The aim of this study is to evaluate the association between ABI with vascular calcifications (VC) and with mortality, in haemodialysis (HD) patients. METHODS We studied 219 HD patients (60% male...

objectives: this paper reviews the current literature on acquired brain injury (abi) with a focus on abi burden, importance of community integration, and community integration definitions suggested by the literature. methods: literature review results: acquired brain injury (abi) is referred to a diverse range of disabilities resulted of injury in different parts of the brain. people with abi a...

Los marcadores moleculares son una herramienta de gran utilidad para estudios diversidad genética, que permite identificar poblaciones con características genéticas particulares, soportan el establecimiento programas conservación y mejoramiento genético. El objetivo este estudio fue evaluar grado información generada por un panel 30 microsatélites en la población avícola Rustipollos. Se obtuvie...

The authors aimed to determine differences in the prevalence of peripheral arterial disease (PAD) and its associations with cardiovascular disease (CVD) risk factors, using different methods of calculating the ankle-brachial index (ABI). Using measurements taken in the bilateral brachial, dorsalis pedis, and posterior tibial arteries, the authors calculated ABI in 3 ways: 1) with the lowest ank...

Abnormally low and high ankle-brachial indices (ABIs) are associated with high cardiovascular morbidity and mortality in patients with chronic kidney disease (CKD), but the mechanisms responsible for the association are not fully known. This study is designed to assess whether there is a significant correlation between abnormal ABI and echocariographic parameters in patients with CKD stages 3-5...

BACKGROUND The associations of low (<0.90) and high (>1.40) ankle brachial index (ABI) with risk of all-cause and cardiovascular disease (CVD) mortality have not been examined in a population-based setting. METHODS AND RESULTS We examined all-cause and CVD mortality in relation to low and high ABI in 4393 American Indians in the Strong Heart Study. Participants had bilateral ABI measurements ...

Recurrent translocation t(10;11) has been reported to be associated with acute myeloid leukemia (AML). Recently, two types of chimeric transcripts, MLL-AF10 in t(10;11)(p12;q23) and CALM-AF10 in t(10;11)(p13;q14), were isolated. t(10;11) is strongly associated with complex translocations, including invins(10;11) and inv(11)t(10;11), because the direction of transcription of AF10 is telomere to ...