Adenosine released during cardiac ischemia exerts a potent, protective effect in the heart. A newly recognized adenosine receptor, the A3 subtype, is expressed on the cardiac ventricular cell, and its activation protects the ventricular heart cell against injury during a subsequent exposure to ischemia. A cultured chicken ventricular myocyte model was used to investigate the cardioprotective ro...

Single domain antibodies (sdAbs) are the recombinantly-expressed variable domain from camelid (or shark) heavy chain only antibodies and provide rugged recognition elements. Many sdAbs possess excellent affinity and specificity; most refold and are able to bind antigen after thermal denaturation. The sdAb A3, specific for the toxin Staphylococcal enterotoxin B (SEB), shows both sub-nanomolar af...

I’ll now sketch a proof. It basically follows by by recalling the definition of the probability of an even conditioned on X2 = a2. We first note that the set of choices for (X 1, X2, 3) given that X2 = a2 is def Sa2 = {(X1, a2, X3) : (X1, a2) ∈ C12 and (a2, X3) ∈ C23} . Conditioning on X2 = a2, we obtain a sample chosen uniformly from Sa2 . Thus, for (a 1, a2, a3) ∈ Sa2 , 1 P [(X1, X2, X3) = (a...

We describe a series of exhaustive computer searches to determine the maximum number of words in ternary codes of length 10 and minimum distance 7, and to find all such optimal codes. We show that there are exactly 10 inequivalent (10, 14, 7) ternary codes. We prove A3(10, 7) = 14 by showing that none of the (10, 14, 7)3 codes can be extended to a (10, 15, 7)3 code, thereby also giving A3(11, 7...

This report provides an overview of the achievements of working group A3 for bringing personalization functionality to the Semantic Web. It continues the work started in the deliverable A3-D1 which gave an overview on personalization know-how of partners in A3. In the deliverable at hand, we report on our achievements on reasoning for personalization in the Semantic Web (see Section 2), and par...

Cyclic nucleotide-gated (CNG) channels in retinal photoreceptors play a crucial role in vertebrate phototransduction. The ligand sensitivity of photoreceptor CNG channels is adjusted during adaptation and in response to paracrine signals, but the mechanisms involved in channel regulation are only partly understood. Heteromeric cone CNGA3 (A3) + CNGB3 (B3) channels are inhibited by membrane phos...

Antigen-specific immunotherapy may offer a unique approach to fight cancer. We have demonstrated that specific immunotherapeutic regimens involving recombinant melanoma antigen family A3 (MAGE-A3) and different immunostimulants exert clinical anticancer activity. In particular, the combination of recombinant MAGE-A3 and AS15, a multicomponent immunostimulant, was found to elicit robust antigen-...

WSS25 is a sulfated polysaccharide that inhibits angiogenesis. However, the mechanism underlying the regulation of angiogenesis by WSS25 is not well understood. Using microRNA (miRNA) microarray analysis, a total of 25 miRNAs were found to be upregulated and 12 (including miR-210) downregulated by WSS25 in human microvascular endothelial cells (HMEC-1). Interestingly, Dicer, a key enzyme for mi...

A motif specific to peptides that bind to the human class I major histocompatibility complex molecule HLA-A3 was identified by sequence analysis of HPLC fractions containing endogenous peptides. Twenty-six different sequences were obtained, 19 of which were nonamers. The majority of these endogenous peptide sequences contained Leu at position (P)2, while most sequences contained Tyr or Lys at P...

We have identified a series of 1H-imidazo-[4,5-c]quinolines as selective allosteric enhancers of human A3 adenosine receptors. Several of these compounds potentiated both the potency and maximal efficacy of agonist-induced responses and selectively decreased the dissociation of the agonist N6-(4-amino-3-[125I]iodobenzyl)-5'-N-methylcarboxamidoadenosine from human A3 adenosine receptors. There w...