Intracerebral inoculation of JHM virus (JHMV), the neuropathic strain of mouse hepatitis virus, into Wistar Furth, Wistar Lewis, and Fischer 344 rats at various ages indicated that Wistar Furth rats are more susceptible to the virus than are the other strains. Fischer 344 and Wistar Lewis rats were more resistant to inoculation at 2 and 5 days of age and completely resistant by 10 days of age. ...

Dry eye disease can cause ocular surface inflammation that disrupts the corneal epithelial barrier. While dry eye patients are known to have an increased risk of corneal infection, it is not known whether there is a direct causal relationship between these two conditions. Here, we tested the hypothesis that experimentally-induced dry eye (EDE) increases susceptibility to corneal infection using...

Moistened barley was inoculated with 1.4 x 10(3) and 1.4 x 10(5) spores, respectively, from ochratoxin A-producing strains of Aspergillus ochraceus and Penicillium varidicatum. To estimate fungal tissue in the barley, the amount of glucosamine was followed for 28 days at 10 and 25 degrees C. Ochratoxin A was also followed during the same period and under the same conditions. The data show that ...

Anaplasma marginale (A. marginale) is a worldwide pathogen that infects variety ofruminants, but mostly cattle. The present study aimed to describe an isolation technique for A. marginale, using chicken embryo Þ broblast (CEF) cell culture. Blood and tick samples were collected from 5 calves 2 3 months old, which considered be infected with A.marginale due anemia, jaundiced mucous membranes, pr...

Inoculation alfalfa seeds with the right Rhizobium strain (nitrogen-fixing bacteria) can stimulate root nodulation and improve plant growth yield. Different inoculation methods are used to inoculate seeds; however, effect of these on germination is not well documented. In present study, was evaluated after using different methods, i.e. Arabic gum solution glucose as adhesive substances surface ...

Mosquito inoculation is a highly sensitive technique for isolation and titration of dengue virus (DENV) from sera, human tissues, wild animals, or mosquitoes. It has been under utilized since it was described 40 years ago because most dengue laboratories do not have access to an insectary to rear mosquitoes. This technique requires good eye-hand coordination while doing manipulation under a ste...

Ocular lesions are a common manifestation of toxoplasmosis in cats. In this survey, 10 healthy Syrianmice were infected through intraperitoneal inoculation of bradyzoites of Toxoplasma gondii (Tehran strain).Mice were killed 30 days after inoculation and the suspension of their brains was prepared, after confirmationof the infection. Seven kittens were fed with the infected mice brain suspensio...

ocular lesions are a common manifestation of toxoplasmosis in cats. in this survey, 10 healthy syrianmice were infected through intraperitoneal inoculation of bradyzoites of toxoplasma gondii (tehran strain).mice were killed 30 days after inoculation and the suspension of their brains was prepared, after confirmationof the infection. seven kittens were fed with the infected mice brain suspensio...