INTRODUCTION ..................................................................................... 446 I. THE CURRENT TECHNOLOGY AND DETECTION ......................... 450 A. Current Technology ............................................................ 450 1. Add-ons, Plug-ins, Extensions, and Apps ..................... 450 2. Accessibility Programs .............................................

We used a mixed 454 and Illumina sequencing strategy. Genomic DNA from each House Finch strain was first nebulized and blunt-end ligated with Roche adaptors. Emulsion PCR was performed using the bulk library and these products were pyrosequenced on Roche 454 Gene Sequencer using FLX chemistry, using physical separation of isolates. Genomic DNA from the four newly sequenced poulty strains (Table...

UNLABELLED PREMISE OF THE STUDY Microsatellite markers were developed and cross-species transferability assessed for two Persoonia species to evaluate genetic diversity and population genetic structure of these broadly distributed southwest Australian tree species. • METHODS AND RESULTS Microsatellite-enriched libraries and 454 GS-FLX shotgun sequencing were used to identity nine microsate...

Next generation sequencing (NGS) technologies have greatly changed the landscape of transcriptomic studies of non-model organisms. Since there is no reference genome available, de novo assembly methods play key roles in the analysis of these data sets. Because of the huge amount of data generated by NGS technologies for each run, many assemblers, e.g., ABySS, Velvet and Trinity, are developed b...

Massively parallel sequencing holds great promise for expression profiling, as it combines the high throughput of SAGE with the accuracy of EST sequencing. Nevertheless, until now only very limited information had been available on the suitability of the current technology to meet the requirements. Here, we evaluate the potential of 454 sequencing technology for expression profiling using Droso...

Although the Compositae harbours only two major food crops, sunflower and lettuce, many other species in this family are utilized by humans and have experienced various levels of domestication. Here, we have used next-generation sequencing technology to develop 15 reference transcriptome assemblies for Compositae crops or their wild relatives. These data allow us to gain insight into the evolut...

Current practices for the therapy of chondrosarcoma, including wide-margin surgical resection and chemotherapy, are less than satisfactory. Recently, emerging evidence has demonstrated that long non-coding RNAs (lncRNAs) have an essential role in the initiation and progression of tumors. As a typical lncRNA, HOTAIR is significantly overexpressed in various tumors. However, the function and pote...

Recension av: Korkiakangas, Pirjo; Olsson, Pia; Ruotsala, Helena & Åström, Anna-Maria (red.) 2016. Kirjoittamalla kerrotut. Kansatieteelliset kyselyt tiedon lähteinä. (Ethnos-toimite 19) Suomen kansatieteilijöiden yhdistys Ethnos ry. Helsinki. 454 s.

Objective(s): Pioglitazone, an anti-diabetic agent, has been widely used to treat type II diabetes. However, the effect of pioglitazone on myocardial ischemia reperfusion injury (MIRI) is still unclear. Herein, the objective of this study is to learn about the regulation and mechanism of pioglitazone effects on oxygen glucose deprivation (OGD)-induced myocardial cell injury.Materials and Method...

Acknowledgement First of all, I would like to thank Prof. Dr. Burkhart Schraven for giving me the opportunity to work in his institute, providing an international, motivating and enthusiastic atmosphere as well as for his strong scientific guidance. I would like to thank my supervisor Dr. Luca Simeoni for his nearly four years of supervision, and the many useful comments and suggestions I have ...