Radiation treatment in patients with head and neck tumors commonly results in hyposalivation and xerostomia due to the loss of fluid-secreting salivary acinar cells. Patients develop susceptibility to oral infections, dental caries, impaired speech and swallowing, reducing the quality of life. Clinical management is largely unsatisfactory. The development of a tissue-engineered, implantable sal...

It is well recognized that one of the major drawbacks of using traditional two dimensional cultures to model the living systems is inaccurately reflecting the physiological manner in which modulators, nutrients, oxygen, and metabolites are applied and removed. Moreover, the two dimensional culture system poorly reflects how different cell types interact with each other in the same microenvironm...

background : human wharton’s jelly mesenchymal stem cells (hwjmscs) express liver-specific markers such as albumin, alpha-fetoprotein, cytokeratin-19, cytokeratin-18, and glucose-6-phosphatase. therefore, they can be considered as a good source for cell replacement therapy for liver diseases. this study aimed to evaluate the effects of various culture systems on the hepatocyte-specific gene exp...

Cell culture based assays have been a wellestablished and important method for the study of tumor cell behavior. The basis for tumor cell behavior, interactions and signaling have all been studied using in vitro cell culture techniques. This has also been the corner stone of drug discovery assays. Typically, cell culturebased assays have been performed as 2-dimensionsional (2D) monolayer cultur...

introduction: radiation imaging is one of the applicable methods in diagnostic medicine and nondestructive testing for industrial applications. in nondestructive 3d imaging, in addition to the radiation source, there is a requirement for a suitable detection system, data acquisition system, mechanical sections for moving objects, reconstruction algorithm and finally a computer for processing an...

backgrounds: evidence is accumulating to support disruption of tissue architecture as a powerful event in tumor formation. for the past four decades, intensive cancer research with the premise of “cancer as a cell based-disease” focused on finding oncogenes or tumor suppressor genes. however, the role of the tissue architecture was neglected. three dimensional (3d) cell cultures which can recap...

Neural progenitor cells (NPCs) derived from human induced pluripotent stem cells (iPSCs) are traditionally maintained and proliferated utilizing two-dimensional (2D) adherent monolayer culture systems. However, NPCs cultured using this system hardly reflect the intrinsic spatial development of brain tissue. In this study, we determined that culturing iPSC-derived NPCs as three-dimensional (3D) ...

Cell culture is an important tool for biological research. Two-dimensional cell culture has been used for some time now, but growing cells in flat layers on plastic surfaces does not accurately model the in vivo state. As compared to the two-dimensional case, the three-dimensional (3D) cell culture allows biological cells to grow or interact with their surroundings in all three dimensions thank...

In this study, we developed a novel method for fabricating microwell arrays constructed from alginate gels, and the alginate gel microwells were used for three-dimensional (3D) cell culture. The alginate gel microwells were fabricated on a patterned ITO electrode using alginate gel electrodeposition. Embryonic stem (ES) cells or hepatocellular carcinoma cells (HepG2) were cultured in the algina...

In this paper, we describe a microfluidic platform that enables three dimensional cell culture within defined microenvironments towards a study of axon-glia interaction. The microdevice can offer both 3D axon isolation from cell body and 3D spatial cell separation between neuron and astrocyte. It consists of large fluidic channels for media supply and small channels for collagen filling as a th...