In the present work, direct enzyme-catalysed esterification of medium chain fatty acids (MCFA) from three different sources (Medium chain triacylglycerols, MCT; saponified MCT and a mixture of free MCFA) was evaluated to obtain structured mono- and diacylglycerols. The esterifications were carried out mixing the different sources of MCFA with glycerol at two weight ratios (1:1 and 4:1, w/w), us...

The molecular mechanisms that enable yeast cells to detect and transmit cold signals and their physiological significance in the adaptive response to low temperatures are unknown. Here, we have demonstrated that the MAPK Hog1p is specifically activated in response to cold. Phosphorylation of Hog1p was dependent on Pbs2p, the MAPK kinase (MAPKK) of the high osmolarity glycerol (HOG) pathway, and...

A slow interconversion between two enzyme forms of rat liver glucokinase has been previously inferred from kinetic assay studies. Two different conformations of the enzyme-substrate complex have now been directly demonstrated by an intrinsic fluorescence enhancement in glucokinase upon addition of glucose. The transition between these two conformations upon glucose addition is measurably slow i...

The research aims to observe the effect of konjac glucomannan and different types plasticizers on microstructure whey film. film was investigated using an experimental method. conducted in descriptive methods with two treatments.The treatment used concentration (P4= 2.5 %; P7= 3 %) (C1= glycerol 30 polyethylene glycol %). results showed that combination had addition concentration, % glycerol, P...

Incubation of rat adipocytes with 1 microM-noradrenaline caused a decrease in both the N-ethylmaleimide-sensitive (microsomal) and N-ethylmaleimide-insensitive (mitochondrial) glycerol phosphate acyltransferase activities measured in homogenates from freeze-stopped cells. The effects of noradrenaline on glycerol phosphate acyltransferase activity were apparent over a wide range of concentration...

- verapamil produces a sustained contraction in isolated biventer-cervicis muscle of chickens between 2-8 days old. from cumulative dose-response curves, ed50 of was calculated for this effect of verapamil. when isolated chicken biventer-cervicis muscle was electrically stimulated, verapamil had no effect on twitch contractures but increased the base line tone of the muscle. glycerol treatment ...

glycerol is a promising feedstock for microbial cultivation and production of 1,3 propanediol (1,3 pdo). here we report a newly isolated bacterial strain ba11 from soil, capable of fermenting glycerol to 1,3 pdo, and has been identified to be a strain of klebsiella pneumoniae. strain ba11 was fast growing showing peak 1,3 pdo production in 6 h of cultivation with productivity of 1.2 g/l-h witho...

A three-dimensional central composite design was used to study the effect of glycerol level, freeze rate, and thaw rate on acrosomal retention and percent motility. Glycerol levels examined were: 1, 4, 7, 10 and 13%. Average freeze rates between 1 0 and 8 0 C examined were: 6.5, 13.1, 26.3, 52.5 and 105.0 C/minute. Thaw rates studied were achieved by plunging straws into water at 5 C for 2 rain...

On the basis of knowledge of the biological role of glycerol in the redox balance of Saccharomyces cerevisiae, a fermentation strategy was defined to reduce the surplus formation of NADH, responsible for glycerol synthesis. A metabolic model was used to predict the operating conditions that would reduce glycerol production during ethanol fermentation. Experimental validation of the simulation r...

Abstract Catalytic valorization of raw glycerol derived from biodiesel into high-value chemicals has attracted great attention. Here, we report chemoenzymatic cascade reactions that convert to lactic acid and glycolic acid. In the enzymatic step, a coenzyme recycling system was developed 1,3-dihydroxyacetone (DHA) with yield 92.3% in potassium phosphate buffer (300 mM, pH 7.1) containing 100 mM...