A spectrophotometric method for the quantitative determination of an enzyme activity resulting in the accumulation of 4-substituted phenols is described in this article. Toluene-4-monooxygenase (T4MO) activity in whole cells of Pseudomonas mendocina KR1 is used to demonstrate this method. This spectrophotometric assay is based on the coupling of T4MO activity with tyrosinase activity. The 4-sub...

A validate rapid, economical and sensitive visible spectrophotometric method has been developed for quantitative determination of amprenavir in bulk drug and formulation samples. This method is validated for irinotecan with chromogenic reagent 3-methyl benzothiazolinone hydrazine (MBTH). The calibration curve was linear over Beer’s concentration range of 25-350 μg/ml. The relative standard devi...

for the determination of serum or plasma triglycerides. The samples are extracted with 2propanol containing activated alumina. An AutoAnalyzer Sampler II and Proportioning Pump II are used in combination with a Gilford Model 300 N spectrophotometer and Model 3091-A debubbler flow cell. Following on-line saponification, glycerol is oxidatively cleaved with sodium metaperiodate. The resulting for...

The antitumor agent thiocoraline is a nonribosomally biosynthesized bisintercalator natural product, which contains in its peptidic backbone two S-methylated l-cysteine residues. S-Methylation occurs very rarely in nature, and is observed extremely rarely in nonribosomal peptide scaffolds. We have proposed that during thiocoraline biosynthesis, TioN, a stand-alone adenylation domain interrupted...

ABSTRACT A simple, economical, accurate, precise and reproducible UV-Visible spectrophotometric method for the routine estimation of dasatinib has been developed. The method is based on the formation of a bluish green colored complex by dasatinib in presence of MBTH reagent. The developed colored complex showed λmax at 630 nm. Beer’s law in the concentration range of 10 to 60 μg/ ml. Results of...

A new series of pyrazolo[3,4-d]pyrimidines has been synthesized. The new compounds were tested for their antitumor activity on 60 different cell lines, and some of the compounds were found to have potent antitumor activity. In particular, 2-hydroxybenzaldehyde [1-(4-chlorophenyl)-3-methyl-1H-pyrazolo-[3,4-d]pyrimidin-4-yl]hydrazone (VIIa) was found to be the most effective among the other deriv...

In the title hydrazone compound, C(14)H(10)FN(3)O(3), the dihedral angle between the two substituted benzene rings is 13.7 (3)°. The mol-ecule exists in a trans configuration with respect to the central methyl-idene unit. In the crystal, mol-ecules are linked through inter-molecular N-H⋯O hydrogen bonds, forming chains along the a axis.

Natural and synthetic derivatives of benzo-γ-pyrones (i.e. flavones, chromones, and coumarins) and their synthetic analogues possess a wide range of biological properties in vitro and in vivo. In this paper we investigated the influence of two hydrazone compounds of chromones, 3-{[(2-dimethoxytiophosphoryl)-2-methylhydrazono]-methyl}-chromen-4-one (CH-3) and 2-amino-6-chloro-3-[(2-hydroxyethyl)...

The new cis-dioxomolybdenum (VI) complexes [MoO2(L2)(H2O)] (2) and [MoO2(L3)(H2O)] (3) containing the tridentate hydrazone-based ligands (H2L2 = N'-(3,5-di-tert-butyl-2-hydroxybenzylidene)-4-methylbenzohydrazide H2L3 N'-(2-hydroxybenzylidene)-2-(hydroxyimino)propanehydrazide) have been synthesized characterized via IR, 1H 13C NMR spectroscopy, mass spectrometry, single crystal X-ray diffraction...