ABSTRACT. The purpose of this study was to develop a method for the extraction and determination 2,4-dichlorophenoxyacetic acid (2,4-D) from soil, sediment, water, investigate its stability in water using high-performance liquid chromatography (HPLC). recoveries spiked compound soil samples found be range 80 100%. yield 2,4-D during acidification corresponding amine 93.84-99.98%. A calibration ...

In vivo spectrophotometry showed that the phytochrome concentration in pea epicotyl hooks decreased at a constant rate for 4 hours when the tissue was exposed to continuous red light. Thereafter the rate slowed progressively so that a steady concentration of phytochrome was approached at hour 7. Returning the plants to darkness resulted in an increase in phytochrome due to the apparent synthesi...

The catabolic adaptability demonstrated by microorganisms is important in the bioremediation of polluted environments. 2,4-dichlorophenoxyacetic acid/α-ketoglutarate dioxygenase is the first enzyme in the catabolic pathway for the 2,4dichlorophenoxyacetic acid (2,4-D). In the present study, dioxygenase enzymes were inducible and the serial adaptation of four studied bacterial isolates steadily ...

The influence of daminozide, an inhibitor of gibberellins biosynthesis, on the somatic embryogenesis in immature and mature embryo derived from tissue cultures of wheat was investigated. Low concentrations of daminozide (0.064-0.127 mM) positively affected the somatic embryogenic capacity of immature embryos (cv. ‘Andros’) cultured on the medium supplemented with either 2,4-dichlorophenoxyaceti...

Purified 2,4-dichlorophenoxyacetic acid (2,4-D)/(alpha)-ketoglutarate dioxygenase (TfdA) was shown to use 4-nitrophenoxyacetic acid (K(infm) = 0.89 (plusmn) 0.04 mM, k(infcat) [catalytic constant] = 540 (plusmn) 10 min(sup-1)), producing intensely yellow 4-nitrophenol. This reagent was used to develop a rapid, continuous, colorimetric assay for the detection of TfdA and analogous activities in ...

The Inhalation of Norisodrine Sulfate Dust: L. R. Krasno, et al ..476 Inhibition of Anaphylactic Shock by Acetylsalicylic Acid: Berry Campbell .478 Effects of 2,4-Dichlorophenoxyacetic Acid on Chicks: Melvin K. Bjorn and Henry T. Northen 479 A New Histological Procedure for Whole Tissue Cultures Grown in Plasma: Annabelle Cohen and Charity Waymouth ...... 480 Removal of the Fertilization Membra...

The expression of lipoxygenases (LOXs) is known to be developmentally regulated in soybeans (Glycine max. [L.] Merr.). Hormones have been firmly established as being involved in the growth and developmental processes of a number of plant species. Correlation between the expression of LOXs and the development and germination of soybean embryos suggests that plant hormones may affect the expressi...

Evidence for Acquisition in Nature of a Chromosomal 2,4-Dichlorophenoxyacetic Acid/a-Ketoglutarate Dioxygenase Gene by Different Burkholderia spp. V. GRACE MATHESON, LARRY J. FORNEY,* YUICHI SUWA,† CINDY H. NAKATSU,‡ ALAN J. SEXSTONE, AND WILLIAM E. HOLBEN§ Department of Plant Pathology and Environmental Microbiology, West Virginia University, Morgantown, West Virginia 26506, and Center for Mic...