A novel reversed-phase HPLC method has been developed and validated for the simultaneous determination of 2-phenoxyethanol, methylparaben, ethylparaben and propylparaben preservatives. The method uses a Lichrosorb C8 (150×4.6 mm, 5 µm) column and isocratic elution. The mobile phase consisted of a mixture of acetonitrile, tetrahydrofuran and water (21:13:66, v/v/v), pumped at a flow rate of 1 ml...

OBJECTIVE 1) Assessment of the frequency of contact sensitization to selected excipients of dermatological topical preparations and cosmetics in patients with chronic eczema. 2) Detection of the sources of sensitization. METHODS 1,927 patients with chronic eczema (mean age 44.3 years, 601 males, 1,326 females) were patch tested with selected excipients. RESULTS The main rates of sensitizati...

In this study, the anesthetic effects of 2-phenoxyethanol (2-PE) on possible primary (cortisol level) and secondary (haematological indices and glucose level) stress responses were studied in silver carp (Hypophthalmichthys molitrix). Fish were first exposed to 0.1, 0.3, 0.5, 0.7 and 0.9 mL/L 2-PE, also the time to induction (deep anesthesia) and recovery were measured. At concentrations of 0.5, ...