Background: Vitrification has been usually used as an assisted reproductive technology in animals and humans. This method needs high concentrations of cryoprotectants that can be toxic with high cooling degrees. Then, vitrification could be change histone modifications such as methylation and acetylation can performance as regulatory controls of gene transcription. So, the purpose of the presen...

Background: Arresting in a certain step of development like two-cell stage could be one of the reasons of infertility. The aim of this study is evaluating the effects of ethanol and strontium on growth and development of two-cell arrested mouse embryos. Materials and Methods: The females were coupled with male following superovulation. Positive vaginal plug mice were killed 48 hours after hCG i...

introduction: in the present study, the effects of oral morphine consumption in pregnant female rats on the amygdaloid complex development in the embryos were investigated. methods: female wistar rats weighing 250-300 g (n=15) were divided into control (n = 8) and experimental groups (n = 7). the experimental group received morphine (0.05 mg/ml) in their tap water. on the 19th day of pregnancy,...

the effect of modified vitrification was assessed on cellular development capability in mouse embryos cultured in vitro. in this study, 466 embryos (from zygote to morula stages) were vitrified then thawed embryos have been incubated for in vitro farther development up to blastocyst stage. also, vitrification and thawing procedures were the same for all experimental groups. mouse different embr...

Background: In somatic cell nuclear transfer (SCNT) method of cloning, transferred nucleus should be dedifferentiated from differentiated state to embryonic state. Molecular analysis showed that the reprogramming in the transferred nucleus was incomplete and cloned embryos have epigenetic abnormalities such as high DNA methylations levels. Since methylation in pre-implantation embryos has a cri...

objective: experiments were conducted to find the differences between post-thaw viability and chromosome aberrations in eight-cell mouse embryos at presence of dimethyl sulfoxide (dmso) and 1, 2-propanediol (proh) as croprotectants in different storage durations. materials and methods: in this case-control study, a total number of 720 mouse embryos from about 250 nmri mice were vitrified with 3...

embryonic stem cells (escs) are derived from the inner cell mass (icm) of blastocyst and differentiate into all three embryonic germ layers: ectoderm, endoderm, and mesoderm. in this study, escs are derived from hand made cloning (hmg) blastocysts and their efficiencies compared to escs derived from in vitro fertilization (ivf) embryos. feeder layer was used for escs culture, and culture medium...

The effect of modified vitrification was assessed on cellular development capability in mouse embryos cultured in vitro. In this study, 466 embryos (from zygote to morula stages) were vitrified then thawed embryos have been incubated for in vitro farther development up to blastocyst stage. Also, vitrification and thawing procedures were the same for all experimental groups. Mouse different embr...