نتایج جستجو برای: 12s rrna و 16s rrna

تعداد نتایج: 796213  

ژورنال: :دنیای میکروب ها 0
مجید مقبلی دانشگاه آزاد اسلامی، واحد دامغان، دانشکده علوم زیستی، گروه میکروبیولوژی محمدرضا شفاعتی دانشگاه آزاد اسلامی، واحد دامغان، دانشکده علوم زیستی، گروه میکروبیولوژی

سابقه و هدف: رودوباکتر باکتری گرم منفی، میله ای شکل و فتوتروفیک ارغوانی است که با اکسیداسیون سولفید هیدرژن به گوگرد قادر به حذف این ترکیب از محیط می باشد. هدف از این پژوهش، جداسازی و شناسایی مولکولی رودوباکتر بود. مواد و روش ها: به منظور جداسازی رودوباکتر، نمونه های پساب از استخر بی هوازی تصفیه خانه فاضلاب مهدی شهر جمع آوری و بر روی محیط  فیننیگ i  کشت داده شدند. پس از استخراج dna ژنومی از باکت...

Journal: :تحقیقات مهندسی کشاورزی 0
محمدامین حجازی استادیار پژوهشکده بیوتکنولوژی کشاورزی شمال غرب و غرب کشور پریا مختاری زنوزی دانشجوی گروه بیوتکنولوژی و اصلاح نباتات، دانشکده کشاورزی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات محمود خسرو شاهلی استاد گروه بیوتکنولوژی و اصلاح نباتات، دانشکده کشاورزی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات ابوالفضل برزگری کارشناس پژوهشکده بیوتکنولوژی کشاورزی شمال غرب و غرب کشور حاجیه لطفی کارشناس پژوهشکده بیوتکنولوژی کشاورزی شمال غرب و غرب کشور صولت اسلامی کارشناس پژوهشکده بیوتکنولوژی کشاورزی شمال غرب و غرب کشور سیامک علیزاده

this study utilized molecular methods to identify 22 isolates from traditional dairy products of azerbaijan. pcr was carried out using the universal primers of 16s rdna and a fragment of ~1500bp was amplified. amplified ribosomal dna restriction analysis was performed using bsuri, mspi, hindiii, taqi and bsp143i. ardra results were compared to the restriction profile in enzymatic restriction an...

Journal: :Nucleic acids research 1982
D O Clary J M Goddard S C Martin C M Fauron D R Wolstenholme

Part of the replication origin-containing A+T-rich region of the Drosophila yakuba mtDNA molecule and segments on either side of this region have been sequenced, and the genes within them identified. The data confirm that the small and large rRNA genes lie in tandem adjacent to that side of the A+T-rich region which is replicated first, and establish that a tRNAval gene lies between the two rRN...

Journal: :iranian journal of pharmaceutical sciences 0
fatemeh dabbagh department of pharmaceutical biotechnology, pharmaceutical sciences research center, school of pharmacy, shiraz university of medical sciences, p.o. box 71345-1583, shiraz, iran zahra moradpour department of pharmaceutical biotechnology, pharmaceutical sciences research center, school of pharmacy, shiraz university of medical sciences, p.o. box 71345-1583, shiraz, iran abdollah ghasemian department of pharmaceutical biotechnology, pharmaceutical sciences research center, school of pharmacy, shiraz university of medical sciences, p.o. box 71345-1583, shiraz, iran younes ghasemi department of pharmaceutical biotechnology, pharmaceutical sciences research center, school of pharmacy, shiraz university of medical sciences, p.o. box 71345-1583, shiraz, iran

uricase or urate oxidase (urate:oxygen oxidoreductase, ec 1.7.3.3), a peroxisomal enzyme which is found in many bacteria, catalyzes the oxidative opening of the purine ring of urate to yield allantoin, carbon dioxide, and hydrogen peroxide. in this study, the phylogeny of urate oxidase (uricase) producing bacteria was studied based on gene sequences of 16s rrna and uricase protein. representati...

Journal: :Biology and Fertility of Soils 2021

Abstract Faeces from cows with different milk yield and non-lactating were applied to soil investigate whether N 2 O efflux is related feeding-induced differences in faecal microbiome abundances of nitrification denitrification genes. Fungal 18S-rRNA gene abundance was the highest faeces group. The 18S-rRNA/ergosterol ratio showed a strong positive correlation 18S-rRNA/fungal glucosamine ratio....

Journal: :Genes & genetic systems 1998
M Sumida M Ogata H Kaneda H Yonekawa

The evolutionary relationships among Japanese pond frogs (Rana nigromaculata, R.porosa porosa, and R. p. brevipoda) were investigated by analyzing nucleotide sequences of mitochondrial cytochrome b (cyt b) and 12S rRNA genes. The nucleotide sequences of 444-bp segment of the cyt b gene and 410-bp segment of 12S rRNA gene were determined by the PCR-direct sequencing method using 18 frogs from 13...

2012
Xiaolin Hao Ting Chen

Because of technological limitations, the primer and amplification biases in targeted sequencing of 16S rRNA genes have veiled the true microbial diversity underlying environmental samples. However, the protocol of metagenomic shotgun sequencing provides 16S rRNA gene fragment data with natural immunity against the biases raised during priming and thus the potential of uncovering the true struc...

Journal: :Systematic biology 2007
Joseph Hughes Martyn Kennedy Kevin P Johnson Ricardo L Palma Roderic D M Page

Lice in the genus Pectinopygus parasitize a single order of birds (Pelecaniformes). To examine the degree of congruence between the phylogenies of 17 Pectinopygus species and their pelecaniform hosts, sequences from mitochondrial 12S rRNA, 16S rRNA, COI, and nuclear wingless and EF1-alpha genes (2290 nucleotides) and from mitochondrial 12S rRNA, COI, and ATPases 8 and 6 genes (1755 nucleotides)...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1999
M T Holder M V Erdmann T P Wilcox R L Caldwell D M Hillis

During the period of September 1997 through July 1998, two coelacanth fishes were captured off Manado Tua Island, Sulawesi, Indonesia. These specimens were caught almost 10,000 km from the only other known population of living coelacanths, Latimeria chalumnae, near the Comores. The Indonesian fish was described recently as a new species, Latimeria menadoensis, based on morphological differentia...

Journal: :Human molecular genetics 2009
Justin Cotney Sharen E McKay Gerald S Shadel

Mitochondrial biogenesis is controlled by signaling networks that relay information to and from the organelles. However, key mitochondrial factors that mediate such pathways and how they contribute to human disease are not understood fully. Here we demonstrate that the rRNA methyltransferase-related human mitochondrial transcription factors B1 and B2 are key downstream effectors of mitochondria...

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