Currently, there are many contaminants of concern that need to be accurately determined help assess their potential environmental hazard. Despite increasing interest, yet few occurrence data exist, likely because they present at low levels and in very complex matrices. Therefore, multiresidue analytical methods for determination highly sensitive, selective, robust. Particularly, due the trace t...

This is the supplementary material for the ICML 2013 paper Dependent Normalized Random Measures by the same authors. A. Notation & Preliminary We list some of the notation used in this paper in Table 1 for reminder. A.1. Definitions For completeness, we restate the definition of MNRM and TNRM. We are given a Poisson process on a product space R×Θ×R with intensity measure ν(dw,dθ,da) (we will us...

CRISPR/Cas9-mediated DNA cleavage (CCMDC) is becoming increasingly used for efficient genome engineering. Proto-spacer adjacent motif (PAM) adjacent to target sequence is one of the key components in the design of CCMDC strategies. It has been reported that NAG sequences are the predominant non-canonical PAM for CCMDC at the human EMX locus, but it is not clear whether it is universal at other ...

Hexavalent chromium [Cr(VI)] is a known etiological factor in human lung cancer. Cr(VI) exposure-related lung cancer has a high mutation incidence in the p53 gene. Upon intake in human cells Cr(VI) is reduced to Cr(III), which is able to conjugate with amino acids and consequently form either binary Cr(III)-DNA or ternary Cr(III)-amino acid-DNA adducts. Both binary and ternary Cr(III)-DNA adduc...

BACKGROUND Corynebacterium glutamicum is an important industrial workhorse and advanced genetic engineering tools are urgently demanded. Recently, the clustered regularly interspaced short palindromic repeats (CRISPR) and their CRISPR-associated proteins (Cas) have revolutionized the field of genome engineering. The CRISPR/Cas9 system that utilizes NGG as protospacer adjacent motif (PAM) and ha...

The Next Generation Grids (NGG) expert group has pioneered the vision of “Invisible Grid” whereby the complexity of Grid systems and architectures is hidden to both developers and users. In this new vision, the Grid has a different role: it will not more provide a virtual computing environment but it will be the basis of a more complex service oriented, knowledge-based and collaborative environ...

CONCLUSIONS Results of the current study revealed improved phonation threshold pressure (PTP), normalized mucosal wave amplitude (NMWA), and less contraction of the lamina propria in injured larynges treated with basic fibroblast growth factor (bFGF). OBJECTIVES We investigated the effects of local injection of bFGF for treatment of acute vocal fold injury in a canine model. METHODS Vocal f...

Seven pairs of southern elephant seals (Mirounga leonina) pups and their dams were sampled during the late weaning season among a breeding population of seals on Elephant Island in Antarctica. The blubber of the pups and the milk and blubber of their dams were analyzed for lipid-normalized concentrations of PCBs and organochlorines compounds in order to evaluate the lactational transfer of thes...

The CRISPR/Cas system is an efficient genome-editing tool to modify genes in mouse zygotes. However, only the Streptococcus pyogenes Cas9 (SpCas9) has been systematically tested for generating gene-modified mice. The protospacer adjacent motif (PAM, 5'-NGG-3') recognized by SpCas9 limits the number of potential target sites for this system. Staphylococcus aureus Cas9 (SaCas9), with its smaller ...

Summary: CRISPRdirect is a simple and functional web server for selecting rational CRISPR/Cas targets from an input sequence. The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA guided by Cas9 nuclease in complex with a guide RNA (gRNA), that complementarily binds to a 20 nt targeted sequence. The target sequence requirement...