The efficacy of random primer-pair arrays compared to conventional RAPD method with a single decamer primer was evaluated using DNA from two species of Cucumis. The banding patterns of amplicons revealed enhanced utility of primer-pair arrays over conventional RAPDs, producing more bands and a higher degree of polymorphism, both at intra- and inter-specific levels. Amplification produced by bot...

  Background: Garlic is a valuable medicinal plant with variability in desirable morphological and physiological characteristics. The analysis of genetic diversity plays an important role in breeding programs. The RAPD technique could be very effective in detecting genetic variation in garlic.  Objective: The objective of the present work was to detect molecular polymorphism among Iranian garli...

Propagation through tissue culture by using orchid seed as explants will produce a lot of plants. This study aims to measure the genetic character plantlets that were regenerated from seeds which have been resulted in vitro culture. The original plants produced was determined Random Amplified Polymorphic DNA (RAPD) molecular markers. results showed primers used RAPD analysis polymorphic band pa...

rapd markers through an application of 23 decamer primers were employed for genetic diversity analysis of some sweet cherry cultivars. bands with good resolution and high repeatability were selected for evaluations. the 23 primers produced 188 bands, among which, 153 were polymorphic. cluster analysis of the cultivars was performed based on the presence (1) and absence (0) of the bands, using j...

Jatropha curcas L. is a rapidly emerging biofuel crop attracting a lot of interest, triggering large investments and rapid expansion of cultivation areas. In the present investigation, the genetic relationships of 29 J. curcas accessions were assessed based on randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses. A total of 72 polymorphic primers (47 RAPD a...

Assessment of environmental contamination on ecology (plant) at molecular and population levels is important in risk quantification and remediation study. Random amplified polymorphic DNA (RAPD) assay and related other fingerprinting techniques have been employed to detect the genotoxin-induced DNA damage and mutations. This research compared the effects occurring at molecular and population le...

in recent years, several plant species have been used as bioindicators, and several tests have been developed to evaluate the toxicity of the environmental contaminants. in this study, the root length and dna band pattern of root tips in maize (zea mays l.) seedlings were exposed at different concentration of cadmium pollutant (40 and 80 mg l−1). the results indicated that the root length of ma...

Nostoc is a diverse genus of filamentous cyanobacteria with tremendous potential for agricultural and industrial applications. Morphometric methods and routine 16S rDNA-based identification undermines the genetic diversity and impedes strain-level differentiation. A comparative study to deduce the discriminatory power of random amplified polymorphic DNA (RAPD) and amplified ribosomal DNA restri...

Genetic analysis in 53 early introduced clones of rubber tree (Hevea brasiliensis) collected from different areas in Southern Thailand was performed using RAPD (Random Amplified Polymorphic DNA) and microsatellite markers. Seventeen cultivated clones (34 samples) were also included to compare DNA patterns. DNA was isolated from leaf samples using CTAB buffer. One hundred and ninety two 10-base ...