The physiological responses supporting freeze tolerance in anurans are well known, but the evolution of this trait remains little studied. This is the first common-garden study of geographic variation in cryoprotective responses to freezing and the degree of freeze tolerance. We studied the gray treefrogs (Hyla versicolor and H. chrysoscelis) from sympatric sites in Minnesota, Indiana and Misso...

Histology and transmission electron microscopy studies were carried out on buffalo muscles that were subjected to repeated freeze-thaw cycles at -10 and -18°C. In the first freeze thaw cycle (-10°C) structures of muscle showed slight change and closely resembled to those of normal muscle. There were frequent gaps in the half way across the fibres and some cracks in individual fibre were also no...

saffron is an important spice values for its color, aroma and flavor in the food industry. a significant proportion of saffron compound is lost during processing and storage because of its volatility and sensitivity to oxidation. thepresent research microencapsulated saffron color compounds (crocin) by freeze drying. the freeze-dried aqueous saffron effective compound extract was first prepared...

The stability of prothrombin and factor VII was studied using accelerated degradation tests in three preparations of freeze-dried pooled normal plasmas. In a previous report (Brozović, Gurd, Robertson, and Bangham, 1971) factor X was shown to be relatively unstable in these preparations of freeze-dried plasma: it was calculated that up to 8% of the original factor X activity would be lost after...

background: preeclampsia is a pregnancy-specific syndrome that may be life-threatening, especially to the fetus. several causes have been reported that may have a possible role in the development of the disorder. interleukin-10 affect maternal intravascular inflammation, as well as endothelial dysfunction. the aim of this study was to investigate the association between il-10 g-1082a polymorphi...

A sensitive PCR based detection of Bacillus anthracis spores from environnment was standardized. Specific 1247bp amplicon could be detected with template concentration as low as 13 pg. Sensitivity was enhanced to 10 fold by nesting with second set of primers, forming 208bp amplicon. Extraction of DNA from spores purified from soil samples by aqueous polymer two-phase system followed by partial ...

SYNOPSIS The effects of nursery practices on freeze tolerance of P taeda and P elliottii seedlings were examined by placing the seedlings in a freezing chamber and lowering the temperature to -10C. Injury was evaluated 10 days after the freeze treatment by examining cambial tissue (browning test). Top-pruning P taeda seedlings (three times) increased freeze tolerance while rootwrenching (six ti...

industrial applications. Advantages of immobilized cell technology include continuous utilization, retention of plasmid-bearing cells, prevention of interfacial inactivation, stimulation of production and excretion of secondary metabolites, and protection against turbulent environment (Melzoch et al., 1994; Nath and Chand, 1996; Tramper, 1990). Potential applications of immobilized cell technol...

We examined the freeze tolerance of bakers' yeast loaded with exogenous trehalose. Freeze-tolerant and freeze-sensitive compressed bakers' yeast samples were soaked at several temperatures in 0.5 M and 1 M trehalose and analyzed. The intracellular trehalose contents in both types of bakers' yeast increased with increasing soaking period. The initial trehalose-accumulation rate increased with in...

This paper provides experimental results to investigate the mechanical properties of sustainable strain-hardening cement composite (2SHCC) for infrastructures after freeze-thaw actions. To improve the sustainability of SHCC materials in this study, high energy-consumptive components-silica sand, cement, and polyvinyl alcohol (PVA) fibers-in the conventional SHCC materials are partially replaced...