Biliary calcium may be a nucleating agent in cholesterol cholelithiasis. A study was designed to determine the effect of binding of ionised calcium on in vitro nucleation time. Ultracentrifuged and microscopically clear gall bladder bile from cholesterol gall stone patients was divided into two aliquots. One aliquot served as control and ionised calcium was bound in the second aliquot by additi...

A modified automated colorimetric micromethod for the determination of red cell 2,3-diphosphoglycerate (2,3-DPG) adapted from that of Grisolia et al (1969) is described. In the modified method, ethylenediaminetetra-acetic acid (EDTA) is not used and consequently concentrations of several reagents are changed. During the development of the method it was found that the presence of EDTA, either in...

A pot study was used to examine the effects of amendments such as EDTA and elemental sulfur on the growth potential, gas exchange features, uptake and mobilization of Pb by wheat (Triticum aestivum L.) in two texturally different contaminated soils at three levels of EDTA (2, 4, 8 mmol kg(-1) dry soil) and two levels of elemental sulfur (100, 200 mmol kg(-1) dry soil). EDTA resulted in more sol...

serum samples measured on both analyzers revealed a proportional difference of 21% for the Immulite analyzer (n ϭ 135; r ϭ 0.97, Spear-man rank correlation). Samples measured in EDTA or cooled EDTA plasma on the Immulite showed differences of 15% and 19%, respectively (n ϭ 135; r ϭ 0.97 and 0.96, Spearman rank correlation), compared with the Immulite 2000. Finally, we tested the in vitro stabil...

Free floating aquatic macrophytes, Lemna minor and Spirodela polyrhiza, were exposed to graded concentration of cadmium in a concentration range 1 to10 mg L for a period of one month to evaluate cadmium accumulation in the presence of EDTA. The EDTA was added at the rate of 1, 2 and 3 mg L separately and the experiment was conducted in triplicate. The water and plant samples were collected at f...

Utilization of genistein as a natural inhibitor was studied alone and in combination with conventional treatments on melanosis and microbial quality of fresh L. vannamei during 10 days ice storage. Treatments were as followed: A (dipping samples in distilled water), B (dipping in 1.25 % sodium metabisulphite solution), C (dipping in 0.01 % genistein + 2% glycerol solution), D (dipping in 0.1 % ...

THE BASIS FOR EDTA-STIMULATION OF METHEMOGLOBIN REDUCTION IN HEMOLYSATES OF HUMAN ERYTHROCYTES Lucy Jean Sannes* and Donald E. Hultquist ** Department of Biological Chemistry The University of Michigan Ann Arbor, Michigan 48109 Received November lo,1979 Summary: We have studied the stimulation by EDTA of methemoglobin reduction in hemolysates of human erythrocytes. The EDTA effect has been show...

BACKGROUND Preanalytical standardization is required for a reliable quantification of the signaling molecules sphingosine-1-phosphate (S1P), sphinganine-1-phosphate (SA1P) and sphingosine (SPH). METHODS Methanolic protein precipitation of 15μL EDTA-plasma was applied prior to analysis. Sphingolipids were separated in 3min by hydrophilic interaction liquid chromatography (HILIC, SeQuant™ ZIC®-...

In this paper, we report on the impact of the structure of ligands on the luminescence enhancement of Eu(III) by directly exciting Eu(III) with visible light in aqueous media. Upon replacing the water molecules that coordinated around a Eu(3+) ion with a ditopic ligand 1,11-bis(2,6-dicarboxypyridin-4-yloxy)-3,6,9-trioxaundecane (L2EO4) or ethylenediaminetetraacetic acid disodium salt (EDTA), si...

Chemically enhanced phytoextraction has been proposed as an effective approach to removing heavy metals from contaminated soil through the use of high biomass plants. Using pot experiments, the effects of the application of EDTA, EDDS and citric acid on the uptake of Cu, Pb, Zn and Cd by corn (Zea mays L. cv. Nongda 108) and bean (Phaseolus vulgaris L. white bean) plants were studied. The resul...