UNLABELLED The search for an efficacious human immunodeficiency virus type 1 (HIV-1) vaccine remains a pressing need. The moderate success of the RV144 Thai clinical vaccine trial suggested that vaccine-induced HIV-1-specific antibodies can reduce the risk of HIV-1 infection. We have made several improvements to the DNA platform and have previously shown that improved DNA vaccines alone are cap...

Bovine brucellosis is a zoonotic disease distributed worldwide and characterized by abortion and reducedfertility in cows. Since brucellosis eradication programme in Iran uses vaccination, test, slaughter andquarantine as control measures, it is essential to distinguish vaccine strains from strains that cause infectionsamong vaccinated cattle herds. We developed and evaluated a multiplex polyme...

Backgrounds: Most of the hepatitis C virus (HCV) infections elicit poor immune responses and 75% to 85% of cases become chronic therefore, the development of an effective vaccine against HCV is of paramount importance. In this study, we aimed to evaluate co-administration of HCV non-Structural Protein 2 and IL-12 DNA vaccines in C57BL/6 mice. Methods: A plasmid encoding full-length HCV NS2 prot...

objective: evaluation of bax encoding plasmid for increasing efficacy of dna vaccine plasmid encoding gb of herpes simplex virus type 1. materials and methods: we compared three different dosages of bax encoding plasmid (pcbax) including 10, 25 and 50 μg of plasmid dna. they were co-injected ineradermally with glycoprotein b (gb) of herpes simplex virus (hsv)-1 encoded plasmid (pcgb) in c57bl/6...

Both cell mediated and humoral immune responses are required for recovery from Brucella abortus infection. In the development of vaccines capable of providing immunity against brucellosis, L7/L12 50S ribosomal protein has been demonstrated to be one of the protective immunogens of Brucella abortus. The recombinant Brucella abortus L7/L12 ribosomal protein previously produced in the E. coli syst...

The potency of naked DNA vaccines is limited by their inability to amplify and spread in vivo. VP22, a HSV-1 protein, has demonstrated the remarkable property of intercellular transport and may thus provide a unique approach for enhancing vaccine potency. Therefore, we created a novel fusion of VP22 with a model Ag, human papillomavirus type 16 E7, in a DNA vaccine that generated enhanced sprea...

BACKGROUND Tyrosine kinase inhibitor gefitinib is effective against lung cancer cells carrying mutant epidermal growth factor receptor (EGFR); however, it is not effective against lung cancer carrying normal EGFR. The breaking of immune tolerance against self epidermal growth factor receptor with active immunization may be a useful approach for the treatment of EGFR-positive lung tumors. Xenoge...

brucellosis is an infectious disease with high impact on innate immune responses which is induced partly by its dna. in the present study the potential differences of wild type and patients isolates versus attenuated vaccine strains in terms of cytokines, ros and no induction on murine splenocytes and peritoneal macrophages were investigated. this panel varied in base composition and included d...

BACKGROUND We have previously described a four antigen malaria vaccine consisting of DNA plasmids boosted by recombinant poxviruses which protects a high percentage of rhesus monkeys against Plasmodium knowlesi (Pk) malaria. This is a multi-stage vaccine that includes two pre-erythrocytic antigens, PkCSP and PkSSP2(TRAP), and two erythrocytic antigens, PkAMA-1 and PkMSP-1(42kD). The present stu...