A rapid and reproducible method for the assay of 14C radioactivity in ketone bodies is described, based on the heat lability of acetoacetate and the ability of beta-hydroxybutyrate to quantitatively bind to and elute from an ion exchange resin. Potential interference from 14C activity in the ketone body metabolites (CO2, bicarbonate and acetone) during infusion of 14C-labeled ketone bodies is e...

Traditionally, the ketone body β-hydroxybutyrate (βOHB) has been looked upon as a carrier of energy from liver to peripheral tissues during fasting or exercise. However, βOHB also signals via extracellular receptors and acts as an endogenous inhibitor of histone deacetylases (HDACs). These recent findings support a model in which βOHB functions to link the environment, in this case the diet, an...

The metabolism of FFA and ketone bodies was studied in fasted rats by infusing at a constant rate tracer amounts of FFA-(3)H, beta-hydroxybutyrate-(14)C or acetoacetate-(14)C for periods up to 2 hr. Blood that was removed for analyses was replaced by continuous transfusion. The rates of turnover of FFA, beta-hydroxybutyrate, and acetoacetate in rats fasted for 2 days were, respectively, 3.2, 5....

The state of therapeutic ketosis can be achieved by using the ketogenic diet (KD) or exogenous ketone supplementation. It was suggested previously that the adenosinergic system may be involved in the mediating effect of KD on suppressing seizure activity in different types of epilepsies, likely by means of adenosine A1 receptors (A1Rs). Thus, we tested in the present study whether exogenous ket...

Methyl Ethyl Ketone and Methyl Isobutyl Ketone Not Carcinogenic The April 22, 1993, issue of Environmental Health Perspectives contained a commentary by Legator and Strawn entitled "Public Health Policies Regarding Hazardous Waste Sites and Cigarette Smoking: An Argument by Analogy." Although the article does not include any discussion of either methyl ethyl ketone (MEK) or methyl isobutyl keto...