Lactate dehydrogenase (LDH) X has been isolated in crystalline form from mouse testes. Homogeneity of the protein preparation was established by disc gel electrophoresis, analytical ultracentrifugation, and immunochemical analyses. LDH-X is clearly distinct in amino acid composition from LDH-1 and LDH-5 with respect to numbers of residues of leucine, glycine, threonine, and methionine. When 6 t...

Lactate dehydrogenase (LDH) X has been isolated in crystalline form from mouse testes. Homogeneity of the protein preparation was established by disc gel electrophoresis, analytical ultracentrifugation, and immunochemical analyses. LDH-X is clearly distinct in amino acid composition from LDH-1 and LDH-5 with respect to numbers of residues of leucine, glycine, threonine, and methionine. When 6 t...

INTRODUCTION AND OBJECTIVES In patients with chronic obstructive pulmonary disease (COPD), lactate dehydrogenase (LDH) levels in skeletal muscles are normal or tend to be elevated; on exercise, these levels increase more rapidly than in individuals without COPD. As it is likely that concentrations of LDH isozymes LDH(4) and LDH(5) are elevated in such patients, we measured those isozymes in per...

An anomalous zymogram of lactate dehydrogenase (LDH) in the serum from a patient with liver cirrhosis was reported. Agar-gel electrophoresis of serum showed an extra LDH band close to the anodic side of LDH5 and a wide band of LDH5. Gel filtration of patient's serum in Sephadex G-200 demonstrated an abnormal LDH fraction eluted between immunoglobulin G (IgG) and macroglobulin in addition to a n...

Upon immunization with LDH-III (subunit composition AABB) rabbits produce anti-A and anti-B antibodies in comparable amounts. These antibodies fit equally well to the hybrid enzyme and to LDH-V (AAAA) or LDH-I (BBBB) respectively, as tested by passive hemagglutination inhibition. No antibodies reacting with both LDH-I and LDH-V were detected. A minority of hybrid-specific antibodies was, howeve...

Both functional overload and hindlimb disuse induce significant energy-dependent remodeling of skeletal muscle. Lactate dehydrogenase (LDH), an important enzyme involved in anaerobic glycolysis, catalyzes the interconversion of lactate and pyruvate critical for meeting rapid high-energy demands. The purpose of this study was to determine rat soleus LDH-A and -B isoform expression, mRNA abundanc...

BACKGROUND Increased levels of lactic dehydrogenase (LDH) in the cerebrospinal fluid (CSF) have been reported in association with several intracranial pathologies. No studies have been performed on patients with Guillain-Barré syndrome (GBS). AIMS To study LDH isoenzymes in CSF of children with GBS. METHODS CSF samples collected from nine patients with GBS were analysed for total LDH isoenz...

A protein with low LDH activity compared to recrystallized muscle LDH and one which appeared to be homogeneous by all available tests for protein homogeneity has been obtained in crystalline form from bovine and rabbit lenses. These lens LDH preparations appeared to be identical immunologically in the other mammalian species examined. The bovine lens LDH had half the molecular weight of LDH iso...

BACKGROUND Lactate formation is up-regulated in tumorous cells by lactate dehydrogenase (LDH). High serum LDH level is linked to many malignancies with poorer survival, but tumour LDH-5 has not been well investigated in non-small cell lung cancer (NSCLC). PATIENTS AND METHODS In 89 patients operated on for NSCLC stage I-III, the serum LDH level was assayed and immunohistochemistry for tumour ...