Regulation of the MYC oncogene remains unclear. Using 10058-F4, a compound that inhibits MYC-MAX transcription factor, MYC protein and gene expression were down-regulated in Namalwa cells, a Burkitt lymphoma. Compound 10058-F4 decreased MYC mRNA (45%), MYC protein (50%), and cell growth (32%). MYC-MAX transcription factor was disrupted 24 h after treatment, resulting in transcriptional inhibiti...

Gallbladder bile of the bullfrog, R a m catesbezana was found to contain a number of minor bile acids and bile alcohols in unconjugated forms. The following bile acids and bile alcohols were identified by combined gas-liquid chromatography-mass spectrometry: cholic acid, allocholic acid, 3a,7a, 12a-trihydroxy-5a-cholestan-26-oic acid, 3a,7a, 12atrihydroxy-24-methyl-5~-cholestan-26-oic acid, 3a,...

In this study, TaTEF-7A, a member of the transcript elongation factor gene family, and its flanking sequences were isolated. TaTEF-7A was located on chromosome 7A and was flanked by markers Xwmc83 and XP3156.3. Subcellular localization revealed that TaTEF-7A protein was localized in the nucleus. This gene was expressed in all organs, but the highest expression occurred in young spikes and develop...

OBJECTIVES MicroRNA-7 (miR-7) is highly connected to cancerous cell proliferation and metastasis. It is also involved in myocardial ischemia-reperfusion (I/R) injury and is upregulated in cardiomyocyte under simulated I/R (SI/R). We aimed to investigate the role of miR-7 during myocardial I/R injury in vitro and in vivo and a possible gene target. METHODS AND RESULTS Real-time PCR revealed th...

The above‑described results demonstrate a genetic association between let‑7a and PCa, supporting a potential role for let‑7a in human PCa. A recent study showed that let‑7a remained to significantly discriminate normal tissues from PCa patients from those of the cancer negative control group with elevated prostate‑specific antigen levels (P < 0.05). 8 In any case, reduced transcription patterns...

We examined the role of microRNAs (miRNAs) in targeting the stromal-derived factor 1α/CXCR4 (SDF-1α/CXCR4) axis to overcome chemoresistance of AML cells. Microarray analysis of OCI-AML3 cells revealed that the miRNA let-7a was downregulated by SDF-1α-mediated CXCR4 activation and increased by CXCR4 inhibition. Overexpression of let-7a in AML cell lines was associated with decreased c-Myc and BC...

Microglia have multiple functions in cerebrovascular and neurodegenerative diseases. Regulation of microglial function during inflammatory stress is important for treatment of central nervous system (CNS) diseases because microglia secrete various substances that affect neurons and glia. MicroRNA-Let-7a (miR-Let-7a) is a tumor suppressor miRNA that has been reported to target transcripts that e...

moclobemide is a selective and reversible monoamine oxidase-a inhibitor, which is used as an antidepressant. three moclobemide analogues were synthesized by replacing moclobemide phenyl ring with substituted imidazoles. so, n-[(4-morpholinyl) ethyl)]-1-benzyl-2-(alkylthio)-1h-imidazole-5-carboxamides (7a-c) were synthesized and studied for the antidepressant activity using forced swimming test ...