background: q fever is a zoonotic disease caused bycoxiella burnetii, a species of bacteria that is distributedworldwide. in cattle, coxiella burnetii infections are generallyasymptomatic but can also be associated with reproductivedisorders. objectives: the aim of this study was to achievemolecular detection of coxiella burnetii in dairy bovine milkfarms using nested pcr in qom province, iran....

methods: we examined 170 formalin-fixed paraffin-embedded samples from esophageal squamous cell carcinoma patients. all subjects live in mazandaran province, a region with high incidence rate of esophageal cancer and have become known as the “asian esophageal cancer belt”. samples were tested for hpv-dna by my09/11 and gp5+/6+ general primers using nested pcr. results: of the 170 escc samples, ...

background : the protozoan parasites cryptosporidium spp. and giardia are known to occur widely in both raw and drinking waters. they are two of the causative agents of waterborne out-breaks of gastroenteritis throughout the world. in the present study, a pcr assay and fa were developed for detection of cryptosporidium oocysts and giardia cyst in environmental samples. methods : we have detecte...

conclusions our study demonstrated that the b1 gene, more so than the re gene, showed positive samples and can be used to detect toxoplasmosis, although the b1 gene, in comparison to the re gene, did not show any superiority of molecular diagnosing capability. results also showed that toxoplasma molecular detection methods can be used instead of routine serological detection methods in a clinic...

INTRODUCTION Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. METHODS We developed the OTsn-PCR assay for the detection of P. brasiliensis in clinical specimens and compared it with other PCR methods. RESULTS The OTsn-PCR assay was positive for all clinical samples, and the detection limit was better or ...