Culture media for mouse preimplantation development are usually derived from two basic solutions: Krebs-Ringer and Tyrode. We have used outbred mice (OF1) to make a comparative analysis of derived media of both types and their success in sustaining development from the one-cell to the blastocyst stage. The best results (up to 50%), in terms of overcoming the two-cell block and sustaining develo...

BACKGROUND Caffeine increases the CAMP production that stimulates spermatozoa movement. Caffeine is also used for induction of in vitro acrosome reaction in mammalian spermatozoa, an important step in achieving fertilization. OBJECTIVE The aim of this study was to assess the effect of caffeine on sperm's motility, vitality and laboratory fertilization rates in mouse in two T6 and M16 media. ...

The effect of organic manure and chemical fertilizers on vegetable crops and soil properties in the radish-stem amaranth-Indian spinach cropping pattern was studied in a homestead area of Gazipur district in Bangladesh. There were eight treatments poultry manure (PM) 5 t ha (T1), cowdung (CD) 10 t ha -1 (T2), household waste (HW) 10 t ha -1 (T3), PM 2.5 t ha -1 + reduced RDF (recommended dose o...

The purpose of this study was to investigate the effect of the level of injury on the serum level of norepinephrine (Nor) and epinephrine (Epi) at rest and after maximal exercise in individuals with paraplegia. Twenty-six male spinal cord-injured subjects with complete paraplegia for at least 9 months were divided into two groups of 13 subjects each according to the level of injury, i.e., T1-T6...

BACKGROUND Clinical appearance and evolution of Canine Leishmaniosis (CL) are the consequence of complex interactions between the parasite and the genetic and immunological backgrounds. We investigated the effect of an immune-modulating diet in CL. Dogs were treated with anti- Leishmania pharmacological therapy combined with standard diet (SD Group) or with the immune-modulating diet (IMMD Grou...

3,5,6-Trichloro-2-pyridinol (TCP) is a widespread pollutant. Some bacteria and fungi have been reported to degrade TCP, but the gene clusters responsible for TCP biodegradation have not been characterized. In this study, a fragment of the reduced flavin adenine dinucleotide (FADH2)-dependent monooxygenase gene tcpA was amplified from the genomic DNA of Ralstonia sp. strain T6 with degenerate pr...

BACKGROUND Opioid analgesics are commonly added to epidural local anesthetics to improve analgesia during surgery. OBJECTIVES The goal of this study was to evaluate the maternal and neonatal effects of adding different doses of tramadol to 2% lidocaine in the epidural anesthesia for cesarean section. PATIENTS AND METHODS Ninety pregnant patients who were candidates for cesarean section unde...

Supervised physical exercise and diet are normally suggested for preventing eventual weight gain, especially in cancer patients. However, little data is available on the effects of “unsupervised” mixed exercises associated with simple correct nutritional advice. This study aims to assess the impact of these two aspects in reducing major risk factors. 37 surviving cancer patients including breas...