Cell pastes and supernatant Escherichia coli samples, taken from an industrial bioprocess overproducing recombinant a2 IFN were analysed using pyrolysis mass spectrometry (PyMS) and diffuse reflectance-absorbance Fourier transform infrared spectroscopy (FT-IR). PyMS and FT-IR are physico-chemical methods which measure predominantly the bond strengths of molecules and the vibrations of bonds wit...

Atmospheric analysis by open-path Fourier-transform infrared (OP/FT-IR) spectrometry has been possible for over two decades but has not been widely used because of the limitations of the software of commercial instruments. In this paper, we describe the current state-of-the-art of the hardware and software that constitutes a contemporary OP/FT-IR spectrometer. We then describe advances that hav...

16 Introduction Lubrication is a vital component of machinery and, as with any component, early detection of wear is essential for timely replacement, avoiding unnecessary maintenance while reducing the risk of catastrophic breakdowns. Oil inevitably degrades with use and is exposed to various sources of contamination, both internal and external, which can lead to its degradation and reduced ef...

The vibrational wavenumbers of sulphaguanidine were calculated using Gaussian03 software at different levels and compared with experimentally observed data. The predicted infrared intensities, Raman activities and first hyperpolarizability are reported. The calculated geometrical parameters (DFT) are in agreement with that of similar derivatives. The potential energy scan studies for different ...

Polypyrrole nanoparticles were synthesized by chemical polymerization method using ammonium persulphate as an oxidant. The synthesized polypyrrole nanoparticles were characterized by FT-IR, UV-Visible, FESEM and CV. The structure of polypyrrole nanoparticles were confirmed by FT-IR spectroscopy. The UV-Vis spectra of polypyrrole nanoparticles show π-π* and n-π* transitions. The morphological pr...

www.spec t roscopyon l i ne . com T he concept of step-scan mid-infrared inter ferometr y dates back to the 1960s when the first optoelectronically controlled step-scan interferometer was reported (1). In contrast to the linear change of the optical path difference (retardation) in a conventional continuous-scan interferometer, the retardation changes stepwise in a step -scan Fourier transform–...