Clostridium perfringens iota-toxin consists of two separate proteins identified as a cell binding protein, iota b (Ib), which forms high-molecular-weight complexes on cells generating Na(+)/K(+)-permeable pores through which iota a (Ia), an ADP-ribosyltransferase, presumably enters the cytosol. Identity of the cell receptor and membrane domains involved in Ib binding, oligomer formation, and in...

introduction and aims: omeprazole is a gastric parietal cells proton pump inhibitor that is also active against h. pylori in vitro. this study was designed to examine the neutralization of h. pylori cytotoxicity on vero cells by omeprazole micronized in strains isolated from gastritis, ulcer, cancer and barrett's ulcer, to determine whether omeprazole can inhibit vacuolation of the vero cells i...

objective: this study was carried out to examine the effect of vero cells co-culture on developmental competence of immature oocytes. materials and methods: bovine cumulus-oocyte-complexes (cocs) were matured in either presence or absence of vero cells. matured oocytes were inseminated and cultured for up to nine days. cleavage percentages were recorded on day two post insemination (pi). embryo...

The role of the cell nucleus in the development of African swine fever virus in Vero cells has been studied. No viral growth could be detected in enucleated cells under conditions that allow normal development of Sindbis virus. Furthermore, African swine fever virus DNA synthesis was inhibited more than 95% after infection of enucleated Vero cells as compared with normal cells.

Introduction: Inactivated influenza vaccines are traditionally produced in chicken embryonated eggs but its limitations in producing the required doses in pandemic outbreaks quickly enough has made searching for alternative modes of production necessary. The use of cell culture-based vaccine production is one way of overcoming the limitations of the egg-based method and securing a more rapid re...

BACKGROUND Renal epithelial cell injury facilitates crystal adhesion to cell surface and serves as a key step in renal stone formation. However, the effects of cell injury on the adhesion of nano-calcium oxalate crystals and the nano-crystal-induced reinjury risk of injured cells remain unclear. METHODS African green monkey renal epithelial (Vero) cells were injured with H2O2 to establish a c...

Serum-free culture is now becoming the general trend of biological production. Its application in Vero cell culture is significant. In the present paper, we reviewed factors affecting Vero cell serum-free culture and several culture techniques. In light of these reviews, we outlined its extensive application prospect.

Abstract: Peganum harmala L. (Zygophylaceae) has been used in a variety of practical applications in medical science. Our objective in the current study was to determine the effects of the seed extract on Herpes simplex virus type 1 (HSV-1) replication in Vero cells. Different concentrations (100, 500, 571, 667, 800 and 1000 µg/ml) of the extract were examined. Experiments were carried ...

Background: Wild-type measles virus (MV) is isolated in B95a but not in Vero cells. Through an adaptation process of wild-type MV to Vero cells, several amino acid substitutions were reported. Methods: Six strains were adapted to Vero cells and membrane (M), fusion (F) and hemagglutinin (H) genes were sequenced. Cell fusion was assessed and recombinant MVs were constructed, having wild-type H o...