In an attempt to isolate virus particles, a hamster macrophage cell line was examined by electron microscopy. This cell line was established from hamster macrophages infiltrating human lymphoid cells heterotransplanted in Syrian hamsters. The cultured cells appeared to be macrophages, containing round smooth nuclei with clear nucleoli, and numerous cytoplasmic primary lysosomes, polysomes, and ...

Virus-like particles (VLPs) can be used as powerful nanoscale weapons to fight against virus infection. In addition to direct use as vaccines, VLPs have been extensively exploited as platforms on which to display foreign antigens for prophylactic vaccination and immunotherapeutic treatment. Unfortunately, fabrication of new chimeric VLP vaccines in a versatile, site-specific and highly efficien...

Ventricular Late Potentials (VLPs) are low-level highfrequency signals that are usually found within the terminal part of the QRS complex from patients after Myocardial Infarction. Patients with VLPs are at risk of developing Ventricular Tachycardia, which is the major cause of death of patients suffering from heart disease. In this study, the Continuous Wavelet Transform was used to detect VLP...

Variations in lipopolysaccharide (LPS), a bacterial outer membrane component, determine virulence of the obligate intracellular bacterium Coxiella burnetii, but the underlying mechanisms are unknown. We find that while avirulent C. burnetii LPS (avLPS) stimulates host p38α-MAPK signaling required for proper trafficking of bacteria containing compartments to lysosomes for destruction, pathogenic...

Nanobiomaterials such as enveloped virus-like particles with specificity can serve a dual function of vaccination and drug delivery system. Here, we expressed colon carcinoma cell-targeting chimeric virus-like particles (VLPs) made using two capsid proteins, gag and M1 from influenza virus A/swine flu/Iowa/15/30/H1N1 in silkworms. These chimeric VLPs displayed a glycosylphosphatidylinositol-anc...

The virus-like particles (VLPs) of the yeast retrotransposon Ty are genetically, structurally and functionally analogous to retroviral nucleocapsids or cores. Like retroviral cores Ty-VLPs package and possibly promote the enzyme activities for reverse transcription and integration, as well as encapsulating the RNA that is the intermediate in retrotransposition. Here we show that Ty-VLPs assembl...

While baculovirus expression of Gag proteins from numerous retroviruses has led reliably to production of virus-like particles (VLPs), we observed that expression of Rous sarcoma virus Gag failed to produce VLPs. Transmission and scanning electron microscopy analysis revealed that the Gag protein reached the plasma membrane but was unable to correctly form particles. Addition of a myristylation...

2015 Vaccination has been the most effective and economical way of combating the spread of infectious disease. Virus-like particles (VLPs) against human and animal diseases caused by picornaviruses are actively being researched and developed as potential vaccines. Promising immunogenicity and protective efficacy data has been reported for VLPs produced using various expression systems. The tran...

UNLABELLED Virus-like particles (VLPs) built on the Newcastle disease virus (NDV) core proteins, NP and M, and containing two chimeric proteins, F/F and H/G, composed of respiratory syncytial virus (RSV) fusion protein (F) and glycoprotein (G) ectodomains fused to the transmembrane and cytoplasmic domains of the NDV F and HN proteins, respectively, stimulate durable, protective RSV neutralizing...

Abstract Virus-like particles (VLPs) are high-priority antigens with highly ordered repetitive structures, which similar to natural viral particles. We have developed a competitive enzyme-linked immunosorbent assay (cELISA) for detecting antibodies directed against Senecavirus A (SVA). Our utilizes SVA VLPs that were expressed and assembled in an E. coli expression system as the coating antigen...