OBJECTIVES The fibroblast growth factor receptor 3 gene (FGFR3) plays a critical role in cartilage growth-plate differentiation and bony development. It has been shown that 97% of patients with achondroplasia have a G to A transition mutation at position 1138 (c.1138 G>A) of codon 380 of the FGFR3 gene. DESIGN AND METHODS Exon 8 of the FGFR3 gene was analyzed in 40 patients with achondroplasi...

Real time reflectivity measurements with subnanosecond time resolution have been used to determine the reflectivity at the melting temperature RS(Tm) of single crystalline Ge and Si at 514.5 nm. Due to the excellent time resolution and sensitivity achieved in a single exposure experiment, the reflectivity of the solid just before melting could be measured. Values of RS(Tm)50.470 60.006 and RS(T...

To overcome blind spots of an ordinary weather radar which scans horizontally at a high altitude, a weather radar which operates vertically, so called an atmospheric profiler, is needed. In this paper, a K-band radar for observing rainfall vertically is introduced, and measurement results of rainfall are shown and discussed. For better performance of the atmospheric profiler, the radar which ha...

Featured Article: Wittwer CT, Reed GH, Gundry CN, Vandersteen JG, Pryor RJ. High-resolution genotyping by amplicon melting analysis using LCGreen. Clin Chem 2003;49:853– 60. DNA melting (the denaturation of DNA by heat) is a fundamental property of DNA that can be used as an analytical tool. Classic studies of DNA melting monitored the absorbance near 260 nm as the temperature was increased and...

BACKGROUND Heteroduplex scanning techniques usually detect all heterozygotes, including common variants not of clinical interest. METHODS We conducted high-resolution melting analysis on the 24 exons of the ACVRL1 and ENG genes implicated in hereditary hemorrhagic telangiectasia (HHT). DNA in samples from 13 controls and 19 patients was PCR amplified in the presence of LCGreen I, and all 768 ...

The melting transition of finite three-dimensional dust clouds (Yukawa balls) from a solid-like to a liquid-like state is systematically studied with high spatial and temporal resolution of the individual grains by means of stereoscopy. Two different melting scenarios are reported: Melting is induced first by an increase of plasma power, and second by laser-induced heating. The experiments conf...

Genotyping by amplicon melting is a minimalistic approach to geno-typing, requiring only PCR, a fluorescent DNA dye, and single-color acquisition of a melting curve in a closed tube. Multiplex genotyping for the F5 1 [coagulation factor V (proaccelerin, labile factor)] Le-iden (1691GϾA), F2 [coagulation factor II (thrombin)] 20210GϾA, and MTHFR [methylenetetrahy-drofolate reductase (NAD(P)H)] 1...